Chen Xinhua, Ren Zhigang, Li Chengxiang, Guo Fei, Zhou Dianbo, Jiang Jianwen, Chen Xinmei, Sun Jihong, Yao Chenguo, Zheng Shusen
1] The Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, China [2] Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang, 310003, China.
The State Key Laboratory of Power Transmission Equipment &System Security and New Technology, Chongqing University, Chongqing, 400030, China.
Sci Rep. 2015 Apr 30;5:9851. doi: 10.1038/srep09851.
Unresectable hepatocellular carcinoma (HCC) needs locoregional ablation as a curative or downstage therapy. Microsecond Pulsed Electric Fields (μsPEFs) is an option. A xenograft tumor model was set up on 48 nude mice by injecting human hepatocellular carcinoma Hep3B cells subcutaneously. The tumor-bearing mice were randomly divided into 3 groups: μsPEFs treated, sham and control group. μsPEFs group was treated by μsPEFs twice in 5 days. Tumor volume, survival, pathology, mitochondria function and cytokines were followed up. μsPEFs was also conducted on 3 swine to determine impact on organ functions. The tumors treated by μsPEFs were completely eradicated while tumors in control and sham groups grew up to 2 cm(3) in 3 weeks. The μsPEFs-treated group indicated mitochondrial damage and tumor necrosis as shown in JC-1 test, flow cytometry, H&E staining and TEM. μsPEFs activates CD56+ and CD68+ cells and inhibits tumor proliferating cell nuclear antigen. μsPEFs inhibits HCC growth in the nude mice by causing mitochondria damage, tumor necrosis and non-specific inflammation. μsPEFs treats porcine livers without damaging vital organs. μsPEFs is a feasible minimally invasive locoregional ablation option.
不可切除的肝细胞癌（HCC）需要局部消融作为一种根治性或降期治疗方法。微秒脉冲电场（μsPEFs）是一种选择。通过皮下注射人肝细胞癌Hep3B细胞，在48只裸鼠上建立了异种移植肿瘤模型。将荷瘤小鼠随机分为3组：μsPEFs治疗组、假手术组和对照组。μsPEFs组在5天内接受两次μsPEFs治疗。对肿瘤体积、生存期、病理学、线粒体功能和细胞因子进行随访。还对3头猪进行了μsPEFs治疗以确定对器官功能的影响。接受μsPEFs治疗的肿瘤被完全根除，而对照组和假手术组的肿瘤在3周内长至2 cm³。如JC-1试验、流式细胞术、苏木精-伊红染色和透射电子显微镜所示，μsPEFs治疗组显示出线粒体损伤和肿瘤坏死。μsPEFs激活CD56+和CD68+细胞并抑制肿瘤增殖细胞核抗原。μsPEFs通过引起线粒体损伤、肿瘤坏死和非特异性炎症来抑制裸鼠体内HCC的生长。μsPEFs治疗猪肝脏而不损伤重要器官。μsPEFs是一种可行的微创局部消融选择。
