Wieczorek K, Osek J
Pol J Vet Sci. 2015;18(1):101-6. doi: 10.1515/pjvs-2015-0013.
Campylobacter infection is the leading foodborne bacterial gastroenteritis worldwide and the bacteria are frequently isolated from the intestines of chickens. The broiler meat contamination with C. jejuni or C. coli may occur during slaughter processing. The aim of the study was to investigate the prevalence of Campylobacter in poultry flocks and the corresponding broiler carcasses in 15 districts (voivodeships) all over Poland. A total of 128 samples from broiler flocks and the corresponding carcasses were collected between February 2011 and April 2013. The Campylobacter isolation and species identification were performed according to ISO 10272-1 standard and with PCR. It was found that 112 flock (96.5%) were contaminated with campylobacters, either C. jejuni (77 samples; 68.7%) or C. coli (35 flocks; 31.3%). Analysis of the corresponding chicken carcasses tested after chilling revealed that 77 out of 128 (60.2%) samples were positive for Campylobacter, either C. jejuni (58; 75.3%) or C. coli (19; 24.7%). Most of the carcasses were contaminated with the same Campylobacter species as identified in the corresponding flock before slaughter. As tested by PCR, out of the 77 crops with C. jejuni 58 were positive for the same bacterial species. On the other hand, out of the remaining 35 flocks infected with C. coli, only 19 corresponding carcass samples were contaminated with C. coli. In three cases in the slaughtered flocks C. jejuni was identified but in the same carcasses C. coli was found. The opposite findings (flock positive for C. coli but the corresponding carcasses contaminated with C. jejuni) were seen in six voivodeships. It was also observed that several carcass samples were negative for C. jejuni and C. coli although the original flocks were Campylobacter-positive before slaughter (total 36 of the 77 samples; 46.7%). On the other hand, some carcasses were contaminated with Campylobacter although the flocks were negative for these bacteria (9 samples; 11.7%) which may also be due to internal contamination during slaughter of broilers.
弯曲杆菌感染是全球食源性细菌性肠胃炎的主要病因，这种细菌经常从鸡的肠道中分离出来。空肠弯曲杆菌或结肠弯曲杆菌对肉鸡的污染可能发生在屠宰加工过程中。本研究的目的是调查波兰全国15个地区（省）家禽群以及相应肉鸡胴体中弯曲杆菌的流行情况。在2011年2月至2013年4月期间，共采集了128份来自肉鸡群及其相应胴体的样本。弯曲杆菌的分离和菌种鉴定按照ISO 10272-1标准并采用聚合酶链反应（PCR）进行。结果发现，112个鸡群（96.5%）被弯曲杆菌污染，其中空肠弯曲杆菌（77个样本；68.7%）或结肠弯曲杆菌（35个鸡群；31.3%）。对冷却后测试的相应鸡胴体进行分析发现，128个样本中有77个（60.2%）弯曲杆菌检测呈阳性，其中空肠弯曲杆菌（58个；75.3%）或结肠弯曲杆菌（19个；24.7%）。大多数胴体被污染的弯曲杆菌种类与屠宰前相应鸡群中鉴定出的种类相同。经PCR检测，77个感染空肠弯曲杆菌的鸡群中，有58个对同一种细菌呈阳性。另一方面，在其余35个感染结肠弯曲杆菌的鸡群中，只有19个相应的胴体样本被结肠弯曲杆菌污染。在屠宰鸡群中有3例鉴定为空肠弯曲杆菌，但在同一胴体中发现的是结肠弯曲杆菌。在6个省出现了相反的情况（鸡群对结肠弯曲杆菌呈阳性，但相应胴体被空肠弯曲杆菌污染）。还观察到，尽管原始鸡群在屠宰前弯曲杆菌检测呈阳性，但仍有几个胴体样本的空肠弯曲杆菌和结肠弯曲杆菌检测呈阴性（77个样本中共有36个；46.7%）。另一方面，一些胴体被弯曲杆菌污染，尽管鸡群对这些细菌检测呈阴性（9个样本；11.7%），这也可能是由于肉鸡屠宰过程中的内部污染。
