Vizzini Priya, Braidot Matteo, Vidic Jasmina, Manzano Marisa
Department of Agriculture Food Environmental and Animal Sciences, University of Udine, 33100 Udine, Italy.
Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78352 Jouy-en-Josas, France.
Micromachines (Basel). 2019 Jul 27;10(8):500. doi: 10.3390/mi10080500.
Foodborne safety has aroused tremendous research interest in recent years because of a global public health problem. The rapid and precise detection of foodborne pathogens can reduce significantly infection diseases and save lives by the early initiation of an effective treatment. This review highlights current advances in the development of biosensors for detection of spp. and that are the most common causes of zoonosis. The consumption of pathogen contaminated food is responsible for humans hospitalization and death. The attention focused on the recognition elements such as antibodies (Ab), DNA probes and aptamers able to recognize cells, amplicons, and specific genes from different samples like bacteria, food, environment and clinical samples. Moreover, the review focused on two main signal-transducing mechanisms, i.e., electrochemical, measuring an amperometric, potentiometric and impedimetric signal; and optical, measuring a light signal by OLED (Organic Light Emitting Diode), SPR (Surface Plasmon Resonance), and Optical fiber. We expect that high-performance of devices being developed through basic research will find extensive applications in environmental monitoring, biomedical diagnostics, and food safety.
近年来，由于全球性的公共卫生问题，食源性安全引发了巨大的研究兴趣。食源性病原体的快速精确检测能够通过尽早开展有效治疗，显著减少感染性疾病并挽救生命。本综述重点介绍了用于检测[具体病原体名称]和[具体病原体名称]的生物传感器开发的当前进展，这两种病原体是人畜共患病最常见的病因。食用受病原体污染的食物会导致人类住院和死亡。注意力集中在识别元件上，如能够识别来自细菌、食物、环境和临床样本等不同样本中的细胞、扩增子和特定基因的抗体（Ab）、DNA探针和适配体。此外，综述重点关注了两种主要的信号转导机制，即电化学机制，测量安培、电位和阻抗信号；以及光学机制，通过有机发光二极管（OLED）、表面等离子体共振（SPR）和光纤测量光信号。我们期望通过基础研究开发的高性能设备将在环境监测、生物医学诊断和食品安全中得到广泛应用。
