Kotenko M A, Smelisheva L N, Kuznetsov A P, Kiseleva M M
Vopr Pitan. 2014;83(6):18-24.
The purpose of research is to study the influence of emotional stress on the activity of digestive enzymes in blood serum of individuals with different initial tone of the autonomic nervous system. Students' session acted as a model of emotional stress. Based on ritm-cardiografic testing according to the indicators of heart rate variability (HRV) 120 healthy young people (18-22 years old) were divided into 3 groups. Hardware-software complex "Varikard 2.51" has been applied to record the electrocardiography and heart rate variability analysis. Regulatory system tension has been selected as an indicator of autonomic balance index, that did not exceed 30 conventional units in student with vagotonia, ranged from 31 to 120 conventional units in normotonics and values from 121 conventional units characterized sympathicotonia. The interpretation of these parameters was carried out in accordance with the recommendations of the European Cardiology and North-American Society for electrophysiology, as well as the guidelines of Russian authors. The level of serum hydrolytic enzymes: 1 pepsinogen (PG 1), 2 pepsinogen (PG 2), amylase, lipase has been determined, the ratio PG 1/PG 2 was calculated. Investigations were carried out on an empty stomach in the day of workshops and during the examination session (emotional stress). The obtained data were statistically processed by variance analysis using the Student's t test. Individual variations were identified in hydrolytic activity of serum enzymes, depending on the original background level of the autonomic nervous system. It has been found that stress factors lead to opposite changes of digestive enzymes activity of blood serum, which were more pronounced in students with high baseline tone of sympatic nervous system. It has been determined that the amylolytic activity was increased in stress in all groups. A reduction in pepsinogen 2 level and lipolytic activity of blood serum has been showed in the group with simpathotony; while endocrine function of the gastric glands in the parasympatic and normotonic groups in terms of pepsinogen 2 increased.
本研究旨在探讨情绪应激对自主神经系统初始张力不同的个体血清中消化酶活性的影响。以学生考试作为情绪应激模型。基于心率变异性（HRV）指标的节律心电图测试，将120名健康年轻人（18 - 22岁）分为3组。采用“Varikard 2.51”软硬件综合系统记录心电图并进行心率变异性分析。选择调节系统张力作为自主平衡指数指标，迷走神经张力亢进的学生该指标不超过30个常规单位，正常张力者范围为31至120个常规单位，交感神经张力亢进者该指标值大于121个常规单位。这些参数的解读依据欧洲心脏病学和北美电生理学会的建议以及俄罗斯作者的指南。测定了血清水解酶水平：胃蛋白酶原1（PG 1）、胃蛋白酶原2（PG 2）、淀粉酶、脂肪酶，并计算了PG 1/PG 2比值。在研讨会当天空腹以及考试期间（情绪应激状态）进行检测。所得数据采用方差分析和学生t检验进行统计学处理。发现血清酶的水解活性存在个体差异，这取决于自主神经系统的原始背景水平。研究发现，应激因素导致血清中消化酶活性发生相反变化，在交感神经系统基线张力高的学生中更为明显。已确定所有组在应激状态下淀粉酶活性均升高。交感神经张力亢进组血清中胃蛋白酶原2水平降低且脂肪酶活性降低；而副交感神经和正常张力组中，就胃蛋白酶原2而言，胃腺的内分泌功能增强。
