Ivanov A P, Kozlov V G, Klebleeva T D, Ivanova O E, Kiktenko A V
Vopr Virusol. 2014;59(6):39-42.
The results of the construction of the first Russian ELISA system for the quantitative determination of D-antigen of 1-3 poliovirus types in the preparations of inactivated poliovirus vaccines are presented. For the first time, this kind of system is based on the use of specific antibodies of class Y (IgY) from egg yolks of immunized hens. It was shown that this ELISA system is specific, sufficiently sensitive, and can be used for quantitative determination of D-antigen of 1-3 poliovirus types in inactivated poliovirus vaccines.
本文展示了首个用于定量测定灭活脊髓灰质炎疫苗制剂中1-3型脊髓灰质炎病毒D抗原的俄罗斯酶联免疫吸附测定（ELISA）系统的构建结果。这种系统首次基于使用免疫母鸡蛋黄中的Y类特异性抗体（IgY）。结果表明，该ELISA系统具有特异性、足够的灵敏度，可用于定量测定灭活脊髓灰质炎疫苗中1-3型脊髓灰质炎病毒的D抗原。
