Sorbonne Universités, UPMC Univ Paris 06, CNRS, Collège de France, UMR 7574, Chimie de la Matière Condensée de Paris, F-75005 Paris, France.
Sorbonne Universités, UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris F75012, France; INSERM, U968, Paris F75012, France; CNRS, UMR_7210, Paris F75012, France; Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, DHU View Maintain, INSERM-DHOS CIC 1423, Paris F-75012, France.
Acta Biomater. 2015 Aug;22:50-8. doi: 10.1016/j.actbio.2015.04.018. Epub 2015 Apr 27.
Several diseases can lead to opacification of cornea requiring transplantation of donor tissue to restore vision. In this context, transparent collagen I fibrillated matrices have been synthesized at 15, 30, 60 and 90 mg/mL. The matrices were evaluated for fibril organizations, transparency, mechanical properties and ability to support corneal epithelial cell culture. The best results were obtained with 90 mg/mL scaffolds. At this concentration, the fibril organization presented some similarities to that found in corneal stroma. Matrices had a mean Young's modulus of 570 kPa and acellular scaffolds had a transparency of 87% in the 380-780 nm wavelength range. Human corneal epithelial cells successfully colonized the surface of the scaffolds and generated an epithelium with characteristics of corneal epithelial cells (i.e. expression of cytokeratin 3 and presence of desmosomes) and maintenance of stemness during culture (i.e. expression of ΔNp63α and formation of holoclones in colony formation assay). Presence of cultured epithelium on the matrices was associated with increased transparency (89%).
几种疾病可导致角膜混浊，需要移植供体组织以恢复视力。在此背景下，已在 15、30、60 和 90 mg/mL 条件下合成了透明的 I 型胶原原纤维化基质。评估了基质的原纤维组织、透明度、机械性能和支持角膜上皮细胞培养的能力。在 90 mg/mL 支架中获得了最佳结果。在该浓度下，原纤维组织的排列与角膜基质中发现的排列具有一定的相似性。基质的杨氏模量平均值为 570 kPa，非细胞支架在 380-780nm 波长范围内的透明度为 87%。人角膜上皮细胞成功地定植在支架表面，并生成具有角膜上皮细胞特征的上皮（即角蛋白 3 的表达和桥粒的存在），并且在培养过程中保持干性（即 ΔNp63α 的表达和集落形成试验中形成全克隆）。培养的上皮存在于基质上与透明度增加（89%）相关。
