Yao Ping, Cui Min, Li Yan, Deng Yiyun, Wu Hao
From the Departments of *Gastroenterology and †Gastroenterology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region; and ‡Intensive Care Unit and §Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.
Pancreas. 2015 Jul;44(5):799-804. doi: 10.1097/MPA.0000000000000339.
The aim of this study was to examine the effects of rhubarb on intestinal flora and toll-like receptors (TLRs) of intestinal mucosa in rats with severe acute pancreatitis (SAP).
Healthy male Sprague-Dawley rats were randomly allocated into sham-operated surgical model of SAP without or with postoperative rhubarb treatment groups (7 in each group). Rats in with rhubarb group received 10% rhubarb decoction (1 mL/200 g) through tube feeding at every 8 hours during postoperative 24 hours. Serum amylase, amount of intestinal flora, and TLR2/TLR4 messenger RNA expression in intestinal mucosa were tested among 3 groups at postoperative 24 hours.
TLR2 and TLR4 messenger RNA expression levels in intestinal mucosa in SAP without rhubarb group were significantly higher than those in sham-operated or SAP with rhubarb groups (P < 0.05). The amount of intestinal lactobacilli and bifidobacteria in SAP without rhubarb group were significantly fewer than in those sham-operated group (P < 0.05) but not significantly different from those in SAP with rhubarb group (P > 0.05). The amount of intestinal Escherichia coli was relatively higher in SAP group than in sham-operated group (P > 0.05) but lesser in rhubarb treatment group (P > 0.05).
Rhubarb might maintain the intestinal mucosal barrier through regulating intestinal flora and inhibiting intestinal inflammatory response in rats with SAP.
本研究旨在探讨大黄对重症急性胰腺炎（SAP）大鼠肠道菌群及肠黏膜 toll 样受体（TLRs）的影响。
将健康雄性 Sprague-Dawley 大鼠随机分为假手术组、未用大黄治疗的 SAP 手术模型组和术后用大黄治疗的 SAP 手术模型组（每组 7 只）。大黄组大鼠在术后 24 小时内每 8 小时经胃管给予 10%大黄水煎剂（1 mL/200 g）。术后 24 小时检测 3 组大鼠的血清淀粉酶、肠道菌群数量及肠黏膜 TLR2/TLR4 信使核糖核酸表达。
未用大黄的 SAP 组肠黏膜 TLR2 和 TLR4 信使核糖核酸表达水平显著高于假手术组或用大黄的 SAP 组（P < 0.05）。未用大黄的 SAP 组肠道乳酸杆菌和双歧杆菌数量显著少于假手术组（P < 0.05），但与用大黄的 SAP 组相比差异无统计学意义（P > 0.05）。SAP 组肠道大肠杆菌数量相对高于假手术组（P > 0.05），但大黄治疗组较少（P > 0.05）。
大黄可能通过调节肠道菌群和抑制 SAP 大鼠的肠道炎症反应来维持肠黏膜屏障。
