抑制葡萄糖转运蛋白1（GLUT-1）的表达可逆转胃癌细胞MKN45中的瓦伯格效应。
Inhibition of glucose-transporter 1 (GLUT-1) expression reversed Warburg effect in gastric cancer cell MKN45.
作者信息
Zhang Tian-Biao, Zhao Ying, Tong Zhao-Xue, Guan Yi-Fu
机构信息
Department of Biochemistry and Molecular Biology, Preclinical Medicine College, China Medical University Shenyang 110001, Liaoning, P. R. China.
Department of General Surgery, Shengjing Hospital of China Medical University Shenyang 110004, Liaoning, P. R. China.
出版信息
Int J Clin Exp Med. 2015 Feb 15;8(2):2423-8. eCollection 2015.
Abstract
Glucose transporter-1 (GLUT-1) plays critical roles in cancer development and progression. Warburg effect (aerobic glycolysis) contributes greatly to tumorigenesis and could be targeted for tumor therapy. However, published data on the relationship between GLUT-1 and Warburg effect are scarce. In this study, gastric cancer cell, MKN45, was transfected with GLUT-1 shRNA using Lipofectamine 2000. Oxygen consumption, LDH activity, lactate production and cytoplasmic pyruvate were detected after MKN45 cells with GLUT-1 knockdown. In the last, hexokinase 1 (HK1), HK2, and pyruvate kinase M2 (PKM2) expression were detected by using western blot. In this study, we showed that inhibition of GLUT-1 expression reversed Warburg effect in MKN45 cells, and induced apoptosis.
摘要
葡萄糖转运蛋白1（GLUT-1）在癌症发展和进展中起着关键作用。瓦伯格效应（有氧糖酵解）对肿瘤发生有很大贡献，可作为肿瘤治疗的靶点。然而，关于GLUT-1与瓦伯格效应之间关系的已发表数据很少。在本研究中，使用脂质体2000将GLUT-1短发夹RNA转染至胃癌细胞MKN45。在GLUT-1敲低的MKN45细胞中检测氧消耗、乳酸脱氢酶活性、乳酸产生和细胞质丙酮酸。最后，通过蛋白质印迹法检测己糖激酶1（HK1）、HK2和丙酮酸激酶M2（PKM2）的表达。在本研究中，我们表明抑制GLUT-1表达可逆转MKN45细胞中的瓦伯格效应，并诱导细胞凋亡。
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