Zhang Qiang, Zhang Jun-Jian, Han Zhong-Mou
Department of Neurology, Zhongnan Hospital of Wuhan University Wuhan 430071, China.
Department of Neurology, Three Gorges University First Clinical Medical College Yichang 443000, China.
Int J Clin Exp Med. 2015 Feb 15;8(2):2435-40. eCollection 2015.
This work aims to explore the efficacy of Rho kinase inhibitor Fasudil on cognitive impairment induced by chronic cerebral hypoperfusion in rats. A total of 32 male adult Sprague Dawley (SD) rats were randomly divided into three groups: treatment group, control group and sham-operated group for severe carotid artery stenosis model. After two weeks, 8.35 mg/kg Fasudil and physiological saline were intraperitoneally applied twice per day in treatment group and control group, respectively. Morris water maze test was performed in each group to detect the changes of cognitive function and observe the hippocampal pathomorphology in rats after eight weeks. The average escape latency distinctly shortened (P < 0.01) and the percentage of swimming distance in the platform quadrant significantly increased (P < 0.01) in treatment group compared with those at corresponding time points in control group. The rate of carotid artery stenosis in rats had no statistical difference between treatment and control groups (P > 0.05). Fasudil effectively improved hippocampal pathomorphology. Rho kinase inhibitor obviously ameliorated cognitive impairment induced by chronic cerebral hypoperfusion in rats.
本研究旨在探讨Rho激酶抑制剂法舒地尔对大鼠慢性脑灌注不足所致认知功能障碍的疗效。将32只成年雄性Sprague Dawley（SD）大鼠随机分为三组：治疗组、对照组和重度颈动脉狭窄模型假手术组。两周后，治疗组和对照组分别每天腹腔注射8.35 mg/kg法舒地尔和生理盐水两次。八周后，对每组大鼠进行Morris水迷宫试验以检测认知功能变化，并观察大鼠海马的病理形态学。与对照组相应时间点相比，治疗组大鼠的平均逃避潜伏期明显缩短（P < 0.01），平台象限游泳距离百分比显著增加（P < 0.01）。治疗组与对照组大鼠的颈动脉狭窄率无统计学差异（P > 0.05）。法舒地尔有效改善了海马病理形态学。Rho激酶抑制剂明显改善了大鼠慢性脑灌注不足所致的认知功能障碍。
