Ding Weiliang, Jiang Yancai, Jiang Yaping, Zhu Taofeng, Xu Ying, Jiang Wenjie, Zhu Wenjiao, Tang Zhian, Ge Zhijun, Ma Tieliang, Tan Yongfei
Department of Laboratory, The Affiliated Yixing Hospital of Jiangsu University Yixing, Jiangsu, China.
Department of Cardiac & Thoracic Surgery, The Affiliated Yixing Hospital of Jiangsu University Yixing, Jiangsu, China.
Int J Clin Exp Med. 2015 Feb 15;8(2):2476-9. eCollection 2015.
In order to investigate the mechanism of human esophageal Eca109 cells induced by Diosgenin (Dio), the p38 specific inhibitor SB203580 was used to inhibit the expression of p38 and Western blot was employed to detect the effect of SB203580 in Eca109 cells. MTT experiments were executed to detect the proliferation of the cells. Western blot was also applied to find the expression of phosphorylated p38 (p-p38). It is found that SB203580 can inhibit the expression of p38 in human esophageal cell Eca109. After treated with 50 μg/mL of Dio and 10 μg/mL of SB203580, the proliferation of cells showed significantly increase and the apoptosis of cells showed significantly decrease compared with the proliferation in the cells treated with Dio only. Moreover, p-p38 protein level was significantly decreased after treated by the two drugs. It is concluded that Dio may regulate esophageal Eca109 cells through p-p38 pathway.
为了研究薯蓣皂苷元（Dio）诱导人食管Eca109细胞的机制，使用p38特异性抑制剂SB203580抑制p38的表达，并采用蛋白质免疫印迹法检测SB203580对Eca109细胞的作用。进行MTT实验以检测细胞的增殖情况。还应用蛋白质免疫印迹法检测磷酸化p38（p-p38）的表达。结果发现，SB203580可抑制人食管细胞Eca109中p38的表达。与仅用Dio处理的细胞相比，用50μg/mL的Dio和10μg/mL的SB203580处理后，细胞增殖显著增加，细胞凋亡显著减少。此外，两种药物处理后p-p38蛋白水平显著降低。得出结论：Dio可能通过p-p38途径调节食管Eca109细胞。
