Institut GEIST, EA 4021, Biomolécules et thérapies anti-tumorales, Faculté de Pharmacie, Laboratoire de Biochimie, Université de Limoges, 87025, Limoges Cedex, France.
Anal Bioanal Chem. 2010 Oct;398(3):1273-83. doi: 10.1007/s00216-010-4062-4. Epub 2010 Aug 17.
Differentiation therapy could be one strategy for stopping cancer cell proliferation. A plant steroid, diosgenin, is known to induce megakaryocytic differentiation in human erythroleukemia (HEL) cells. In recent studies, the use of sedimentation field-flow fractionation (SdFFF) allowed the preparation of subpopulations that may differ in regard to sensitivity to differentiation induction. The specific goal of this study was to determine the relationship between cell cycle stage and sensitivity to megakaryocytic differentiation induction of HEL cells. After first confirming the capacity of diosgenin to specifically select targets, hyperlayer SdFFF cell sorting was used to prepare fractions according to cell cycle position from crude HEL cells. The sensitivities of these fractions to diosgenin-induced differentiation were then tested. The coupling of SdFFF cell separation to imaging flow cytometry showed that G1-phase cells were more sensitive to differentiation induction than S/G2M-phase cells, confirming the relationship between cell status at the start of induction, the extent of the biological event, and the potential of SdFFF in cancer research.
分化疗法可能是阻止癌细胞增殖的一种策略。一种植物甾体，薯蓣皂素，已知能诱导人红白血病（HEL）细胞向巨核细胞分化。在最近的研究中，使用沉降场流分离（SdFFF）允许制备亚群，这些亚群在对分化诱导的敏感性方面可能有所不同。本研究的具体目标是确定 HEL 细胞的细胞周期阶段与对巨核细胞分化诱导的敏感性之间的关系。在首先证实薯蓣皂素特异性选择靶标的能力后，使用超层 SdFFF 细胞分选根据粗 HEL 细胞的细胞周期位置制备级分。然后测试这些级分对薯蓣皂素诱导分化的敏感性。将 SdFFF 细胞分离与成像流式细胞术相结合表明，G1 期细胞比 S/G2M 期细胞对分化诱导更敏感，这证实了诱导开始时细胞状态、生物学事件的程度以及 SdFFF 在癌症研究中的潜力之间的关系。
