Cetinkunar Suleyman, Tokgoz Serhat, Bilgin Bulent Caglar, Erdem Hasan, Aktimur Recep, Can Serpil, Erol Huseyin Serkan, Isgoren Atilla, Sozen Selim, Polat Yilmaz
Adana Numune Egitim ve Arastirma Hastanesi, Genel Cerrahi Klinigi Adana, Türkiye.
Ankara Diskapi Yildirim Beyazit Egitim ve Arastirma Hastanesi, Genel Cerrahi Klinigi Ankara, Türkiye.
Int J Clin Exp Med. 2015 Feb 15;8(2):2578-85. eCollection 2015.
Silymarin from Silybum marianum was found to reduce liver injury. The aim of the present study was to investigate the effects of silymarin on hepatic regeneration in partially hepatectomized rats.
Thirty Wistar-Albino rats were divided into 3 groups of 10 animals as sham, control and experimental groups. In the sham group (n=10) abdominal incision was closed after laparotomy. In the control group (n=10), the rats underwent 70% hepatectomy after laparotomy. In the experimental group (n=10) after partial 70% hepatectomy, silymarin (200 mg/kg/d) were given to rats for 10 days. Rats in three groups were sacrificed on 10 days. Aspartate (AST) and alanine transaminase (ALT), gamma glutamyl transferase (GGT), ALP, LDH and total bilirubin levels were measured using intracardiac blood samples. Tissue malondialdehyde (MDA) and tissue glutathion (GSH) and Superoxide dismutase (SOD) levels were measured. To reveal the increase in the mass of the remnant liver tissue in the control and experimental groups relative weight of the liver was calculated. Histopathological analysis of the liver was performed using a semi-quantitative scoring system.
A statistically significant difference among three groups was not shown for AST and ALT levels. A statistically significant difference was found between the groups as for total bilirubin and gamma glutamyl transferase levels. Increases in relative liver weights were seen with time in Groups 2 and 3. A statistically significant difference was not found for tissue malondialdehyde, Glutathion and Superoxide dismutase levels between hepatectomy and hepatectomy + silymarin groups. On liver tissue sections of the rats in the hepatectomy + silymarin group, increased regeneration and lipid peroxidation were observed accompanied by decreased antioxidant response.
It has been observed that silymarin with many established functions such as antiproliferative, anti-inflammatory and energy antioxidant effects, does not contributed to proliferative regeneration of the liver-which has very important metabolic functions -after partial hepatectomy; instead it will decrease serum levels of transaminases.
已发现水飞蓟宾可减轻肝脏损伤。本研究的目的是探讨水飞蓟宾对部分肝切除大鼠肝脏再生的影响。
将30只Wistar - 白化大鼠分为假手术组、对照组和实验组，每组10只动物。假手术组（n = 10）剖腹术后缝合腹部切口。对照组（n = 10）大鼠剖腹术后行70%肝切除术。实验组（n = 10）大鼠行70%部分肝切除术后，给予水飞蓟宾（200 mg/kg/d），持续10天。三组大鼠均于第10天处死。采用心内血样测定天冬氨酸（AST）、丙氨酸转氨酶（ALT）、γ - 谷氨酰转移酶（GGT）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）和总胆红素水平。测定组织丙二醛（MDA）、组织谷胱甘肽（GSH）和超氧化物歧化酶（SOD）水平。为揭示对照组和实验组残余肝组织质量的增加，计算肝脏相对重量。采用半定量评分系统对肝脏进行组织病理学分析。
三组之间AST和ALT水平未显示出统计学显著差异。总胆红素和γ - 谷氨酰转移酶水平在各组之间存在统计学显著差异。第2组和第3组肝脏相对重量随时间增加。肝切除组与肝切除 + 水飞蓟宾组之间组织丙二醛、谷胱甘肽和超氧化物歧化酶水平未发现统计学显著差异。在肝切除 + 水飞蓟宾组大鼠的肝组织切片上，观察到再生增加和脂质过氧化，同时抗氧化反应降低。
已观察到具有抗增殖、抗炎和能量抗氧化等多种既定功能的水飞蓟宾，在部分肝切除后对具有非常重要代谢功能的肝脏的增殖性再生没有促进作用；相反，它会降低转氨酶的血清水平。
