Department of Prosthodontics, Tepebasi Oral and Dental Health Hospital, Ankara, Turkey.
Department of Prosthodontics, Faculty of Dentistry, Gazi University, Ankara, Turkey.
J Adv Prosthodont. 2015 Apr;7(2):93-7. doi: 10.4047/jap.2015.7.2.93. Epub 2015 Apr 23.
Xerostomia can diminish the quality of life, leads to changes in normal chemical composition of saliva and oral microbiata, and increases the risk for opportunistic infections, such as Candida albicans. Various artificial salivas have been considered for patients with xerostomia. However, the knowledge on the antifungal and antiadhesive activity of artificial saliva substitutes is limited. The aim of the present study was to evaluate influence of two artificial salivas on the adhesion of Candida albicans to the polymethylmethacrylate disc specimens.
Two commercial artificial salivas (Saliva Orthana and Biotene Oral Balance Gel) were selected. 45 polymethylmethacrylate disc specimens were prepared and randomly allocated into 3 groups; Saliva Orthana, Biotene-Oral Balance gel and distilled water. Specimens were stored in the artificial saliva or in the sterile distilled water for 60 minutes at 37℃. Then they were exposed to yeast suspensions including Candida albicans. Yeast cells were counted using ×40 magnification under a light microscope and data were analysed.
Analysis of data indicated statistically significant difference in adhesion of Candida albicans among all experimental groups (P=.000). Findings indicated that Saliva Orthana had higher adhesion scores than the Biotene Oral Balance gel and distilled water (P<.05).
In comparison of Saliva Orthana, the use of Biotene Oral Balance Gel including lysozyme, lactoferrin and peroxidase may be an appropriate treatment method to prevent of adhesion of Candida albicans and related infections in patients with xerostomia.
口干会降低生活质量，导致唾液正常化学成分和口腔微生物群发生变化，并增加机会性感染（如白色念珠菌）的风险。已经为口干症患者考虑了各种人工唾液。然而，关于人工唾液替代品的抗真菌和抗粘附活性的知识有限。本研究的目的是评估两种人工唾液对白色念珠菌粘附到聚甲基丙烯酸甲酯圆盘标本的影响。
选择了两种商业人工唾液（Saliva Orthana 和 Biotene Oral Balance Gel）。制备了 45 个聚甲基丙烯酸甲酯圆盘标本，并将其随机分配到 3 组；Saliva Orthana、Biotene-Oral Balance gel 和无菌蒸馏水。将标本在 37°C 下分别保存在人工唾液或无菌蒸馏水中 60 分钟。然后，将它们暴露于包含白色念珠菌的酵母悬浮液中。使用 ×40 放大倍数在光显微镜下计数酵母细胞，并分析数据。
数据分析表明，所有实验组的白色念珠菌粘附存在统计学差异（P=.000）。结果表明，Saliva Orthana 的粘附评分高于 Biotene Oral Balance gel 和蒸馏水（P<.05）。
与 Saliva Orthana 相比，使用含有溶菌酶、乳铁蛋白和过氧化物酶的 Biotene Oral Balance Gel 可能是预防口干症患者白色念珠菌粘附和相关感染的一种合适的治疗方法。
