Dept. of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, Yunkeun-Dong 28, Chongro-Ku, Seoul 110-749, Republic of Korea.
Arch Oral Biol. 2012 Jun;57(6):684-8. doi: 10.1016/j.archoralbio.2011.11.007. Epub 2011 Nov 30.
The purpose of this study was to investigate the candidacidal activity of the glucose oxidase-mediated lactoperoxidase system at various levels of glucose and glucose oxidase.
Candida albicans ATCC strains 18804, 10231, 11006, bovine lactoperoxidase (25 and 50 μg/mL), and KSCN (1 mM) were used. Different levels of glucose oxidase (1, 5, 10, and 20 units/mL) and glucose (0.03, 0.3, and 3.0 mg/mL) were added to complete the system. The candidacidal activity of the system was examined by preincubating its components for 0-60 min, and then with C. albicans. Candidacidal activity was determined by comparing the numbers of CFU and calculating the percent loss of cell viability.
The system displayed 13.9-27.4% (without preincubation) to 28.6-34.3% (preincubation for 60 min) loss of viability at 25 μg/mL of bovine lactoperoxidase, 10 units/mL of glucose oxidase, and 0.03 mg/mL of glucose; similar results were obtained with 20 units/mL of glucose oxidase or 0.06 mg/mL of glucose. The candidacidal activity of the system increased markedly as the glucose concentration increased. The candidacidal activity displayed 87.2% (without preincubation) to 100.0% (preincubation for 60 min) at 3.0 mg/mL of glucose. At 3.0 mg/mL of glucose, the system containing 1 or 5 units of glucose oxidase also showed significant levels of candidacidal activity.
The candidacidal activity of the glucose oxidase-mediated lactoperoxidase system at physiological concentrations of salivary glucose was moderate, but was greatly elevated with increases of glucose level.
本研究旨在探讨不同浓度葡萄糖和葡萄糖氧化酶条件下，葡萄糖氧化酶介导的乳过氧化物酶系统的杀菌活性。
使用白念珠菌 ATCC 菌株 18804、10231、11006、牛乳过氧化物酶（25 和 50μg/ml）和 KSCN（1mM）。分别添加不同浓度的葡萄糖氧化酶（1、5、10 和 20 单位/ml）和葡萄糖（0.03、0.3 和 3.0mg/ml）来完成该系统。通过预孵育系统成分 0-60 分钟，然后与白念珠菌孵育，来检测系统的杀菌活性。通过比较 CFU 数量并计算细胞活力丧失的百分比来确定杀菌活性。
在 25μg/ml 牛乳过氧化物酶、10 单位/ml 葡萄糖氧化酶和 0.03mg/ml 葡萄糖条件下，该系统未经预孵育时表现出 13.9-27.4%（无预孵育）至 28.6-34.3%（预孵育 60 分钟）的存活率丧失，而在 20 单位/ml 葡萄糖氧化酶或 0.06mg/ml 葡萄糖条件下得到了类似的结果。随着葡萄糖浓度的增加，系统的杀菌活性显著增加。在 3.0mg/ml 葡萄糖条件下，未经预孵育时系统的杀菌活性达到 87.2%（无预孵育），预孵育 60 分钟时达到 100.0%。在 3.0mg/ml 葡萄糖条件下，含有 1 或 5 单位葡萄糖氧化酶的系统也表现出显著的杀菌活性。
在唾液葡萄糖的生理浓度下，葡萄糖氧化酶介导的乳过氧化物酶系统的杀菌活性适中，但随着葡萄糖水平的升高而大大提高。
