Department of Materials Science and Engineering, Vanung University, Chung-Li, Taiwan.
Department of Chemical Engineering, Ming Chi University of Technology, New Taipei City 243, Taiwan.
Carbohydr Polym. 2015 Aug 1;126:97-107. doi: 10.1016/j.carbpol.2015.02.068. Epub 2015 Mar 14.
In this study, a fucoidan-shelled chitosan bead was developed with the purpose of oral delivery of berberine to inhibit the growth of bacteria. The cross-linking level and swelling property of the beads were affected by the pH value and the composition of the genipin/fucoidan combined gelling agent. The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4). Furthermore, a nanoparticles/beads complex system was developed by incorporation of berberine-loaded chitosan/fucoidan nanoparticles in the fucoidan-shelled chitosan beads. The nanoparticles/beads complex served as a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h. Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
本研究制备了一种以岩藻聚糖硫酸酯壳聚糖珠为载体的载黄连素口服给药系统，以抑制细菌生长。珠的交联水平和溶胀性能受 pH 值和京尼平/岩藻聚糖复合凝胶剂组成的影响。载黄连素珠在模拟胃液(pH 1.2)中的药物释放速度快于模拟肠液(pH 7.4)。此外，通过将载黄连素壳聚糖/岩藻聚糖纳米粒包埋于岩藻聚糖硫酸酯壳聚糖珠中，开发了纳米粒/珠复合物系统。纳米粒/珠复合物作为药物载体，可在模拟胃液中延迟黄连素的释放，估计时滞为 2 h。研究结果表明，载黄连素珠和纳米粒/珠复合物可有效抑制金黄色葡萄球菌和大肠杆菌等常见临床病原体的生长抑制作用，并具有持续释放黄连素抑制细菌生长超过 24 h 的优势。
