Park Si-Eun, Lee Siwon, Kim Haneul, Ahn Tae-Young
Department of Microbiology, College of Natural Sciences, Dankook University, Cheonan 330-714, Korea.
Environmental Infrastructure Research Department, National Institute of Environmental Research, Incheon 440-170, Korea.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2496-2499. doi: 10.1099/ijs.0.000292. Epub 2015 May 1.
A novel bacterial strain, designated JBR12T, was isolated from sediment of a shallow stream in Cheonan, Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JBR12T belongs to the genus Emticicia, and indicated that its closest relatives are Emticicia oligotrophica DSM 17448T (97.8 % sequence similarity) and E. ginsengisoli Gsoil 085T (94.3%). A DNA-DNA hybridization experiment revealed < 70 % genomic relatedness between strain JBR12T and E. oligotrophica DSM 17448T. The major fatty acids (>5% of the total) were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 3-OH, anteiso-C15 : 0 and iso-C15 : 0 3-OH. The DNA G+C content of strain JBR12T was 37.7 mol%. According to data from the present polyphasic taxonomic study, strain JBR12T represents a novel species of the genus Emticicia, for which the name Emticicia sediminis sp. nov. is proposed. The type strain is JBR12T ( = KACC 17466T = JCM 19321T).
从韩国天安一条浅溪的沉积物中分离出了一株新的细菌菌株，命名为JBR12T。基于16S rRNA基因序列的系统发育分析表明，菌株JBR12T属于Emticicia属，表明其最接近的亲缘种是寡营养Emticicia菌DSM 17448T（序列相似性为97.8%）和人参Emticicia菌Gsoil 085T（94.3%）。DNA-DNA杂交实验表明，菌株JBR12T与寡营养Emticicia菌DSM 17448T之间的基因组相关性<70%。主要脂肪酸（占总量的>5%）为异-C15:0、特征性总和3（C16:1ω7c和/或C16:1ω6c）、异-C15:0 3-OH、anteiso-C15:0和异-C15:0 3-OH。菌株JBR12T的DNA G+C含量为37.7mol%。根据目前多相分类研究的数据，菌株JBR12T代表Emticicia属的一个新物种，为此提出了新名称沉积物Emticicia菌。模式菌株为JBR12T（=KACC 17466T=JCM 19321T）。
