Ngo Hien T T, Trinh Huan, Yang Jung-Eun, Won Kyung-Hwa, Chu Dong-Hun, Kook MooChang, Yi Tae-Hoo
College of Life Sciences, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.
SD Biotechnologies Co., Ltd. #301 Seoul Hightech Venture Center, 29, Gonghang-daero 61-gil, Ganseo-gu, Seoul 07563, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1703-1708. doi: 10.1099/ijsem.0.001840. Epub 2017 Jun 7.
A Gram-stain-negative, facultatively anaerobic, non-motile, rod-shaped and yellow-pigmented bacterium, designated strain THG-DN6.14T, was isolated from a freshwater sample near Donghaksa temple in Daejeon, South Korea. On the basis of the results of 16S rRNA gene sequence comparisons, THG-DN6.14T was found to be most closely related to Emticicia sediminis JBR12T (99.1 % sequence similarity), Emticicia oligotrophica DSM 17448T (97.6 %), Emticicia aquatica HMF2925T (96.5 %), and Emticicia ginsengisoliGsoil 085T (94.4 %). The DNA-DNA relatedness between THG-DN6.14T and its phylogenetically closest neighbours was below 65.0 %. The DNA G+C content was 43.3 mol%. The major polar lipids were found to be phosphatidylethanolamine, an unidentified glycolipid and an unidentified aminoglycolipid. The major fatty acids were identified as C16 : 0, iso-C15 : 0, iso-C17 : 0 3-OH, and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The respiratory quinone was menaquinone MK-7. These data supported the affiliation of THG-DN6.14T to the genus Emticicia. THG-DN6.14Tcould be distinguished from related species of the genus Emticicia by physiological and biochemical tests. Therefore, the novel isolate represents a novel species, for which the name Emticicia aquatilis sp. nov. is proposed, with THG-DN6.14T (=KACC 18540T=CGMCC 1.15958T) as the type strain.
从韩国大田东鹤寺附近的淡水样本中分离出一株革兰氏染色阴性、兼性厌氧、无运动性、杆状且产黄色色素的细菌，命名为THG-DN6.14T。基于16S rRNA基因序列比较结果，发现THG-DN6.14T与沉积物埃姆蒂西亚菌JBR12T（序列相似性为99.1%）、寡营养埃姆蒂西亚菌DSM 17448T（97.6%）、水生埃姆蒂西亚菌HMF2925T（96.5%）和人参土埃姆蒂西亚菌Gsoil 085T（94.4%）关系最为密切。THG-DN6.14T与其系统发育关系最密切的近缘菌株之间的DNA-DNA相关性低于65.0%。DNA G+C含量为43.3 mol%。主要极性脂类为磷脂酰乙醇胺、一种未鉴定的糖脂和一种未鉴定的氨基糖脂。主要脂肪酸鉴定为C16 : 0、异-C15 : 0、异-C17 : 0 3-OH和特征性总和3（C16 : 1ω7c和/或C16 : 1ω6c）。呼吸醌为甲基萘醌MK-7。这些数据支持THG-DN6.14T归属于埃姆蒂西亚菌属。通过生理生化试验，THG-DN6.14T可与埃姆蒂西亚菌属的相关物种区分开来。因此，该新分离株代表一个新物种，提议将其命名为水生埃姆蒂西亚菌（Emticicia aquatilis sp. nov.），模式菌株为THG-DN6.14T（=KACC 18540T=CGMCC 1.15958T）。
