Chen Qing, Zang Xiao-Xia, Hang Xing, Wang Hong-Mei, Jia Wei-Bin, Li Shun-Peng, Jiang Jian-Dong, Chen Kai
Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, PR China.
College of Life Sciences, Zaozhuang University, 277160 Zaozhuang, PR China.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2885-2890. doi: 10.1099/ijsem.0.002041. Epub 2017 Aug 18.
Bacterial strain ZZ-4T, a Gram-stain-negative, aerobic, non-spore-forming, non-motile, non-flagellated, rod-shaped bacterium, was isolated from tetrabromobisphenol A-contaminated soil in PR China. The taxonomic position of this strain was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain ZZ-4T was a member of the genus Emticicia and showed the highest sequence similarity to Emticicia fontis IMCC1731T (98.0 %) and Emticicia ginsengisoli Gsoil 085T (97.2 %), and lower (<97 %) sequence similarity to other known Emticicia species. Chemotaxonomic analysis revealed that strain ZZ-4T possessed menaquinone MK-7 as the major isoprenoid quinone; and iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7), iso-C17 : 0 3-OH and C16 : 1ω5c were the predominant fatty acids. Strain ZZ-4T showed low DNA-DNA relatedness with E. fontis IMCC1731T (39.8±3.1 %) and E. ginsengisoli Gsoil 085T (44.51±1.5 %). The DNA G+C content was 38.3 mol%. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data and DNA-DNA hybridization results, strain ZZ-4T is considered to represent a novel species of the genus Emticicia, for which the name Emticicia soli sp. nov. is proposed. The type strain is ZZ-4T (=KCTC 52344T=CCTCC AB 2016137T).
细菌菌株ZZ-4T是从中国受四溴双酚A污染的土壤中分离得到的革兰氏阴性、需氧、不形成芽孢、无运动性、无鞭毛的杆状细菌。采用多相分类方法对该菌株的分类地位进行了研究。基于16S rRNA基因序列的系统发育分析表明，菌株ZZ-4T是Emticicia属的成员，与Emticicia fontis IMCC1731T（98.0%）和Emticicia ginsengisoli Gsoil 085T（97.2%）的序列相似性最高，与其他已知的Emticicia属物种的序列相似性较低（<97%）。化学分类分析表明，菌株ZZ-4T以甲基萘醌MK-7作为主要类异戊二烯醌；异-C15 : 0、特征性总和3（C16 : 1ω6c和/或C16 : 1ω7）、异-C17 : 0 3-OH和C16 : 1ω5c是主要脂肪酸。菌株ZZ-4T与E. fontis IMCC1731T（39.8±3.1%）和E. ginsengisoli Gsoil 085T（44.51±1.5%）的DNA-DNA相关性较低。DNA G+C含量为38.3 mol%。基于系统发育和表型特征、化学分类数据以及DNA-DNA杂交结果，菌株ZZ-4T被认为代表Emticicia属的一个新物种，为此提出新名称Emticicia soli sp. nov.。模式菌株为ZZ-4T（=KCTC 52,344T=CCTCC AB 2016137T）。
