Jang Yeongseon, Jang Seokyoon, Min Mihee, Hong Joo-Hyun, Lee Hanbyul, Lee Hwanhwi, Lim Young Woon, Kim Jae-Jin
School of Biological Sciences, Seoul National University, Seoul, 151-747, Republic of Korea.
Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 136-701, Korea.
Microb Ecol. 2015 Oct;70(3):634-45. doi: 10.1007/s00248-015-0616-5. Epub 2015 May 3.
In this study, three different methods (fruiting body collection, mycelial isolation, and 454 sequencing) were implemented to determine the diversity of wood-inhabiting basidiomycetes from dead Manchurian fir (Abies holophylla). The three methods recovered similar species richness (26 species from fruiting bodies, 32 species from mycelia, and 32 species from 454 sequencing), but Fisher's alpha, Shannon-Wiener, Simpson's diversity indices of fungal communities indicated fruiting body collection and mycelial isolation displayed higher diversity compared with 454 sequencing. In total, 75 wood-inhabiting basidiomycetes were detected. The most frequently observed species were Heterobasidion orientale (fruiting body collection), Bjerkandera adusta (mycelial isolation), and Trichaptum fusco-violaceum (454 sequencing). Only two species, Hymenochaete yasudae and Hypochnicium karstenii, were detected by all three methods. This result indicated that Manchurian fir harbors a diverse basidiomycetous fungal community and for complete estimation of fungal diversity, multiple methods should be used. Further studies are required to understand their ecology in the context of forest ecosystems.
在本研究中，采用了三种不同方法（子实体采集、菌丝体分离和454测序）来确定来自死红松（Abies holophylla）的木生担子菌的多样性。这三种方法所获得的物种丰富度相似（子实体采集得到26种，菌丝体分离得到32种，454测序得到32种），但真菌群落的费希尔阿尔法、香农-维纳、辛普森多样性指数表明，与454测序相比，子实体采集和菌丝体分离显示出更高的多样性。总共检测到75种木生担子菌。最常观察到的物种是东方异担子菌（子实体采集）、烟管菌（菌丝体分离）和暗黄针层孔菌（454测序）。所有三种方法仅检测到两个物种，即安田被毛革菌和卡氏肉座菌。该结果表明，红松含有多样的担子菌真菌群落，为全面评估真菌多样性，应使用多种方法。需要进一步研究以了解它们在森林生态系统背景下的生态学。
