Millar J Cameron, Pang Iok-Hou
North Texas Eye Research Institute (NTERI), University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA; Department of Cell Biology and Immunology, University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.
North Texas Eye Research Institute (NTERI), University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA; Department of Cell Biology and Immunology, University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA; Department of Pharmaceutical Sciences, University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.
Exp Eye Res. 2015 Dec;141:74-90. doi: 10.1016/j.exer.2015.04.018. Epub 2015 Apr 28.
Glaucoma is a leading cause of blindness, which is treatable but currently incurable. Numerous animal models therefore have both been and continue to be utilized in the study of numerous aspects of this condition. One important facet associated with the use of such models is the ability to accurately and reproducibly measure (by cannulation) or estimate (by tonometry) intraocular pressure (IOP). At this juncture there are several different approaches to IOP measurement in different experimental animal species, and the list continues to grow. We feel therefore that a review of this subject matter is timely and should prove useful to others who wish to perform similar measurements. The general principles underlying various types of tonometric and non-tonometric techniques for non-continuous determination of IOP are considered. There follows discussion of specific details as to how these techniques are applied to experimental animal species involved in the research of this disease. Specific comments regarding anesthesia, circadian rhythm, and animal handling are also included, especially in the case of rodents. Brief consideration is also given to possible future developments.
青光眼是导致失明的主要原因之一，虽然可治疗但目前无法治愈。因此，众多动物模型已被用于且仍在被用于该病症诸多方面的研究。使用此类模型的一个重要方面是能够准确且可重复地测量（通过插管）或估计（通过眼压测量法）眼压（IOP）。在这一点上，针对不同实验动物物种的眼压测量有几种不同的方法，而且这一列表还在不断增加。因此，我们认为对这一主题进行综述恰逢其时，并且应该会对其他希望进行类似测量的人有所帮助。文中考虑了用于非连续眼压测定的各种眼压测量和非眼压测量技术的一般原理。接下来讨论了这些技术如何应用于参与该疾病研究的实验动物物种的具体细节。还包括了关于麻醉、昼夜节律和动物处理的具体评论，特别是在啮齿动物的情况下。此外还简要考虑了可能的未来发展。
