Rahaiee Somayeh, Shojaosadati Seyed Abbas, Hashemi Maryam, Moini Sohrab, Razavi Seyed Hadi
Department of Food Science, Engineering and Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, P.O. Box 4111, Karaj 31587-77871, Iran.
Biotechnology Group, Department of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Int J Biol Macromol. 2015 Aug;79:423-32. doi: 10.1016/j.ijbiomac.2015.04.041. Epub 2015 Apr 28.
This study aimed to improve the stability of crocin, a saffron carotenoid, encapsulating into chitosan (Cs)-sodium alginate (Alg) nanoparticles prepared by a modified ionic gelation method were investigated as a new carrier to improve the stability of crocin. Response surface methodology was used to optimize the important variables, namely the concentrations of Cs and Alg, and pH influencing the particle size, zeta-potential, and encapsulation efficiency to find the optimum formulation for production of crocin nanoparticles (CNPs). Microscopic analysis and dynamic light scattering examination indicated non-smooth and spherical nanoparticles with the size range of 165-230 nm in weight ratio of Cs:Alg (1:1.25) and pH 4.7. Fourier transform-infrared spectroscopy displayed an extensive hydrogen bonding interaction between the crocin and biopolymers. Encapsulation efficiency, loading capacity and yield of CNPs were 38.16, 30.96 and 48.33%, respectively. The zeta-potential of NPs was about -33.52 mV which resulted in the better stability of NPs during manipulation and storage. Stability studies showed that nanoencapsulation provided enhanced crocin stability with biopolymers compared to the standard crocin under unfavorable environmental conditions.
本研究旨在提高藏红花类胡萝卜素西红花苷的稳定性，将其包封于通过改良离子凝胶法制备的壳聚糖（Cs）-海藻酸钠（Alg）纳米颗粒中，作为提高西红花苷稳定性的新型载体进行了研究。采用响应面法优化重要变量，即Cs和Alg的浓度以及影响粒径、zeta电位和包封效率的pH值，以找到生产西红花苷纳米颗粒（CNPs）的最佳配方。显微镜分析和动态光散射检测表明，在Cs:Alg重量比为1:1.25且pH值为4.7时，纳米颗粒不光滑且呈球形，粒径范围为165 - 230 nm。傅里叶变换红外光谱显示西红花苷与生物聚合物之间存在广泛的氢键相互作用。CNPs的包封效率、载药量和产率分别为38.16%、30.96%和48.33%。纳米颗粒的zeta电位约为-33.52 mV，这使得纳米颗粒在操作和储存过程中具有更好的稳定性。稳定性研究表明，与标准西红花苷相比，在不利的环境条件下，纳米包封能增强西红花苷与生物聚合物的稳定性。
