从大西洋鲭鱼（Scomber scombrus）副产物的Protamex水解物中纯化和鉴定四种抗菌肽。
Purification and characterization of four antibacterial peptides from protamex hydrolysate of Atlantic mackerel (Scomber scombrus) by-products.
作者信息
Ennaas Nadia, Hammami Riadh, Beaulieu Lucie, Fliss Ismail
机构信息
STELA Dairy Research Centre, Institute of Nutrition and Functional Foods, Université Laval, Québec, QC G1V 0A6, Canada.
STELA Dairy Research Centre, Institute of Nutrition and Functional Foods, Université Laval, Québec, QC G1V 0A6, Canada.
出版信息
Biochem Biophys Res Commun. 2015 Jul 3;462(3):195-200. doi: 10.1016/j.bbrc.2015.04.091. Epub 2015 Apr 28.
Proteins from fish by-product sources are valuable source of bioactive peptides and show promise as functional foods ingredients. The objective of the present study was to isolate and characterize antibacterial peptides from protamex hydrolysates of Atlantic mackerel (Scomber scombrus) by-products. Four sequences SIFIQRFTT (P4), RKSGDPLGR (P8.1), AKPGDGAGSGPR (P8.2) and GLPGPLGPAGPK (P11) were identified in peptide fractions separated using RP-HPLC. At 200 μg mL(-1), while peptides P8.1, P8.2 and P11 exhibited partial inhibition, P4 totally inhibited tested Gram-positive (Listeria innocua) and Gram-negative (Escherichia coli) bacterial strains. These results suggest that the protein hydrolysate derived from mackerel by-products could be used as an antimicrobial ingredient in both functional food and nutraceutical applications.
来自鱼类副产物的蛋白质是生物活性肽的宝贵来源，有望成为功能性食品成分。本研究的目的是从大西洋鲭鱼（Scomber scombrus）副产物的Protamex水解物中分离并鉴定抗菌肽。在使用反相高效液相色谱法分离的肽组分中鉴定出四个序列SIFIQRFTT（P4）、RKSGDPLGR（P8.1）、AKPGDGAGSGPR（P8.2）和GLPGPLGPAGPK（P11）。在200μg mL(-1)时，虽然肽P8.1、P8.2和P11表现出部分抑制作用，但P4完全抑制了测试的革兰氏阳性菌（无害李斯特菌）和革兰氏阴性菌（大肠杆菌）菌株。这些结果表明，鲭鱼副产物衍生的蛋白质水解物可作为抗菌成分用于功能性食品和营养保健品应用中。
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