Kim Ju-Won, Kong Hee Jeong, Nam Bo-Hye, Park Jung Youn, Kim Do-Hyung, Jeong Ji-Min, Park Chan-Il
Biotechnology Research Division, National Institute of Fisheries Science, 408-1 Sirang-ri, Gijang-up, Gijang-gun, Busan 46083, Republic of Korea.
Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University, 45, Yongso-ro, Nam-Gu, Busan, Republic of Korea.
Dev Comp Immunol. 2017 Nov;76:262-267. doi: 10.1016/j.dci.2017.06.004. Epub 2017 Jun 15.
We isolated and characterised a cDNA encoding the lysosomal protective protein (serine protease) cathepsin A (CTSA) from rock bream (Oplegnathus fasciatus). The full-length rock bream CTSA (RbCTSA) cDNA (1814 bp) contains an open reading frame of 1419 bp that encodes 472 amino acids. Alignment of multiple CTSA protein sequences revealed that the active site serine and histidine residues were well-conserved among the other CTSA sequences. RbCTSA is highly expressed in the peripheral blood leukocytes, kidney, spleen, liver, intestine, gill, heart, brain, stomach, and eye. RbCTSA expression was also examined in several tissues, including whole kidneys and spleens, under bacterial and viral challenge. In general, all of the examined tissues that were infected with Edwardsiella tarda, Streptococcus iniae, or red sea bream iridovirus (RSIV) exhibited significant upregulation of RbCTSA expression compared to the controls. Our results reveal that RbCTSA may be involved in the immune responses of rock bream.
我们从条石鲷（Oplegnathus fasciatus）中分离并鉴定了一个编码溶酶体保护蛋白（丝氨酸蛋白酶）组织蛋白酶A（CTSA）的cDNA。条石鲷CTSA（RbCTSA）全长cDNA（1814 bp）包含一个1419 bp的开放阅读框，编码472个氨基酸。多个CTSA蛋白序列的比对显示，活性位点丝氨酸和组氨酸残基在其他CTSA序列中高度保守。RbCTSA在外周血白细胞、肾脏、脾脏、肝脏、肠道、鳃、心脏、大脑、胃和眼睛中高表达。还在细菌和病毒攻击下的几个组织（包括全肾和脾脏）中检测了RbCTSA的表达。总体而言，与对照组相比，所有感染迟缓爱德华氏菌、海豚链球菌或条石鲷虹彩病毒（RSIV）的检测组织中RbCTSA表达均显著上调。我们的结果表明，RbCTSA可能参与条石鲷的免疫反应。
