Department of Prosthodontics, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China; Oral Bioengineering and Regenerative Medicine Lab, Shanghai Research Institute of Stomatology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, 639 Zhizaoju Road, Shanghai 200011, China.
Department of Biomedical Engineering, School of Engineering, Tufts University, 4 Colby St, Medford, MA 02155, USA.
Biomaterials. 2015 Jul;56:68-77. doi: 10.1016/j.biomaterials.2015.03.053. Epub 2015 Apr 15.
Despite the promise for stem cell-based tissue engineering for regenerative therapy, slow and insufficient vascularization of large tissue constructs negatively impacts the survival and function of these transplanted cells. A combination of channeled porous silk scaffolds and prevascularization with endothelial cells was investigated to test the ability of this tissue engineering strategy to support rapid and extensive vascularization process. We report that hollow channels promote in vitro prevascularization by facilitating endothelial cell growth, VEGF secretion, and capillary-like tube formation. When implanted in vivo, the pre-established vascular networks in the hollow channel scaffolds anastomose with host vessels and exhibit accelerated vascular infiltration throughout the whole tissue construct, which provides timely and sufficient nutrients to ensure the survival of the transplanted stem cells. This tissue engineering strategy can promote the effective application of stem cell-based regeneration to improve future clinical applications.
尽管基于干细胞的组织工程在再生治疗方面具有广阔的前景，但大组织工程的血管化速度缓慢且不足，这会对移植细胞的存活和功能产生负面影响。本研究采用带有通道的多孔丝支架与内皮细胞预血管化相结合的方法，探究该组织工程策略支持快速广泛血管化过程的能力。我们的研究表明，中空通道通过促进内皮细胞的生长、VEGF 的分泌和类似毛细血管的管状结构形成，促进体外预血管化。当植入体内时，中空通道支架内预先建立的血管网络与宿主血管吻合，并在整个组织工程中加速血管浸润，为移植的干细胞提供及时、充足的营养，以确保其存活。该组织工程策略可以促进基于干细胞的再生的有效应用，以改善未来的临床应用。
