Liu Rongpu, Wang Guifang, Ma Li, Yang Guangzheng, Lin Sihan, Sun Ningjia, Wang Jiajia, Ma Huijing, Jiang Xinquan, Zhang Wenjie
Department of Prosthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
College of Stomatology, Shanghai Jiao Tong University, Shanghai, China.
Bioact Mater. 2025 Feb 19;48:242-256. doi: 10.1016/j.bioactmat.2025.02.020. eCollection 2025 Jun.
Guided bone regeneration (GBR) is widely applied in implant dentistry, employing barrier membranes to create an osteogenic space by preventing gingival tissue ingrowth. However, this method does not enhance the osteogenic capacity of osteoblasts, limiting sufficient bone volume in larger defects. Inspired by axolotl limb regeneration, abundant soft tissue-derived stem cells mobilized to the defect may facilitate comprehensive osteogenesis within a BMP-2-enriched environment. We developed a biomimetic channel system (BCS) to promote alveolar bone regeneration, using channel structures to activate gingival-derived stem cells under a BMP-2-enriched biological barrier. In a cell-tracing mouse model, Prrx1 stem cells demonstrated a critical role in BMP-2-induced subcutaneous osteogenesis. Sequencing and histological analyses revealed that channel structures significantly enhance soft tissue cell proliferation and migration. Attributable to the biological barrier, BCS applications markedly improved bone formation in beagle mandibular defects. These results suggest a novel osteoinductive strategy for alveolar bone regeneration that functions without a traditional barrier membrane.
引导骨再生（GBR）在种植牙科中被广泛应用，通过使用屏障膜来防止牙龈组织向内生长，从而创造一个成骨空间。然而，这种方法并不能增强成骨细胞的成骨能力，限制了较大骨缺损中足够骨量的形成。受蝾螈肢体再生的启发，动员到缺损处的大量软组织来源干细胞可能在富含骨形态发生蛋白-2（BMP-2）的环境中促进全面的骨生成。我们开发了一种仿生通道系统（BCS）来促进牙槽骨再生，利用通道结构在富含BMP-2的生物屏障下激活牙龈来源的干细胞。在细胞追踪小鼠模型中，Prrx1干细胞在BMP-2诱导的皮下骨生成中发挥了关键作用。测序和组织学分析表明，通道结构显著增强了软组织细胞的增殖和迁移。由于生物屏障的作用，BCS应用显著改善了比格犬下颌骨缺损处的骨形成。这些结果表明了一种用于牙槽骨再生的新型骨诱导策略，可以在不使用传统屏障膜的情况下发挥作用。
