Freise Amanda C, Wu Anna M
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, USA.
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, USA.
Mol Immunol. 2015 Oct;67(2 Pt A):142-52. doi: 10.1016/j.molimm.2015.04.001. Epub 2015 Apr 28.
Antibodies have clearly demonstrated their utility as therapeutics, providing highly selective and effective drugs to treat diseases in oncology, hematology, cardiology, immunology and autoimmunity, and infectious diseases. More recently, a pressing need for equally specific and targeted imaging agents for assessing disease in vivo, in preclinical models and patients, has emerged. This review summarizes strategies for developing and optimizing antibodies as targeted probes for use in non-invasive imaging using radioactive, optical, magnetic resonance, and ultrasound approaches. Recent advances in engineered antibody fragments and scaffolds, conjugation and labeling methods, and multimodality probes are highlighted. Importantly, antibody-based imaging probes are seeing new applications in detection and quantitation of cell surface biomarkers, imaging specific responses to targeted therapies, and monitoring immune responses in oncology and other diseases. Antibody-based imaging will provide essential tools to facilitate the transition to truly precision medicine.
抗体已明确证明其作为治疗药物的效用，为治疗肿瘤学、血液学、心脏病学、免疫学和自身免疫性疾病以及传染病提供了高度选择性和有效的药物。最近，出现了对用于在临床前模型和患者体内评估疾病的同样特异性和靶向性成像剂的迫切需求。本综述总结了开发和优化抗体作为使用放射性、光学、磁共振和超声方法进行无创成像的靶向探针的策略。重点介绍了工程化抗体片段和支架、偶联和标记方法以及多模态探针的最新进展。重要的是，基于抗体的成像探针在细胞表面生物标志物的检测和定量、对靶向治疗的特异性反应成像以及肿瘤学和其他疾病中的免疫反应监测方面有了新的应用。基于抗体的成像将提供必要的工具，以促进向真正的精准医学的转变。
