State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, PR China; Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan 430070, PR China.
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, PR China; Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan 430070, PR China.
Biomaterials. 2015 Jul;55:44-53. doi: 10.1016/j.biomaterials.2015.03.028. Epub 2015 Apr 9.
Peripheral nerve injury requires optimal conditions in both macro-environment and micro-environment for reestablishment. Though various strategies have been carried out to improve the macro-environment, the underlying molecular mechanism of axon regeneration in the micro-environment provided by nerve conduit remains unclear. In this study, the rat sciatic nerve of 10 mm defect was made and bridged by PRGD/PDLLA nerve conduit. We investigated the process of nerve regeneration using histological, functional and real time PCR analyses after implantation from 7 to 35 days. Our data demonstrated that the ciliary neurotrophic factor highly expressed and up-regulated the downstream signaling pathways, in the case of activated signals, the expressions of axon sprout relative proteins, such as tubulin and growth-associated protein-43, were strongly augmented. Taken together, these data suggest a possible mechanism of axon regeneration promoted by PRGD/PDLLA conduit, which created a micro-environment for enhancement of diffusion of neurotrophic factors secreted by the injured nerve stumps, and activation of molecular signal transduction involved in growth cone, to potentiate the nerve recovery.
周围神经损伤需要宏观环境和微观环境的最佳条件才能重建。尽管已经实施了各种策略来改善宏观环境，但神经导管提供的微观环境中轴突再生的潜在分子机制仍不清楚。在这项研究中，通过 PRGD/PDLLA 神经导管桥接大鼠 10mm 缺损的坐骨神经。在植入后 7 至 35 天，我们使用组织学、功能和实时 PCR 分析来研究神经再生过程。我们的数据表明，睫状神经营养因子高度表达并上调下游信号通路，在激活信号的情况下，轴突芽相对蛋白，如微管蛋白和生长相关蛋白-43 的表达强烈增强。总之，这些数据表明 PRGD/PDLLA 导管促进轴突再生的可能机制，该机制为增强由损伤神经末梢分泌的神经营养因子的扩散创造了一个微环境，并激活了参与生长锥的分子信号转导，从而增强神经恢复。
