Zhu Yinbiao, Yang Da, Ren Qian, Yang Yang, Liu Xin, Xu Xiulong, Liu Wei, Chen Sujuan, Peng Daxin, Liu Xiufan
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, PR China; Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease, Yangzhou 225009, PR China.
China Animal Disease Control Center, Beijing 100123, PR China.
Vet Microbiol. 2015 Jul 9;178(1-2):144-9. doi: 10.1016/j.vetmic.2015.04.012. Epub 2015 Apr 23.
H9N2 avian influenza virus (AIV) evolves rapidly in both genovariation and antigenicity. It is essential to monitor the change of antigenicity, in particular in the hemagglutinin (HA) protein. Here we reported the selection of antigenic variants from A/Chicken/Shanghai/F/98 (H9N2) and A/chicken/Taixing/10/2010 (H9N2) viruses using HA-specific monoclonal antibodies (MAbs). Based on the reactivity of these variant and wild-type strains with the MAbs, we identified 6 critical amino acid positions (92, 145, 166, 167, 168, and 197) in the H9 antigenic sites, including the position 92 that has never been reported. Among AIVs originated from chicken in mainland China, the rates of Gly and Arg at position 92 within BJ/94-like (A/chicken/Beijing/1/1994) lineage viruses were 62.2% (28/45) and 37.8% (17/45), respectively; whereas the rates of Gly and Arg at position 92 within Y280-like (A/duck/Hong Kong/Y280/97) lineage viruses were 0.3% (2/670) and 99.1% (673/679), respectively. Our study suggests that G92R mutation together with other identified antigenic sites may serve as molecular markers for H9N2 virus evolution, and may aid improving AIV vaccine effectiveness.
H9N2禽流感病毒（AIV）在基因变异和抗原性方面都快速进化。监测抗原性变化至关重要，尤其是血凝素（HA）蛋白的抗原性变化。在此，我们报告了利用HA特异性单克隆抗体（MAb）从A/鸡/上海/F/98（H9N2）和A/鸡/泰兴/10/2010（H9N2）病毒中筛选抗原变异体。基于这些变异株和野生型毒株与MAb的反应性，我们在H9抗原位点鉴定出6个关键氨基酸位置（92、145、166、167、168和197），其中包括此前从未报道过的92位。在源自中国大陆鸡的AIV中，BJ/94样（A/鸡/北京/1/1994）谱系病毒92位的甘氨酸（Gly）和精氨酸（Arg）比率分别为62.2%（28/45）和37.8%（17/45）；而Y280样（A/鸭/香港/Y2~0/97）谱系病毒92位的Gly和Arg比率分别为0.3%（2/670）和99.1%（673/679）。我们的研究表明，G92R突变连同其他已鉴定的抗原位点可能作为H9N2病毒进化的分子标记，并可能有助于提高禽流感疫苗的有效性。
