两种新型戊型肝炎病毒基因型G5和G6的病毒样颗粒的产生及其与已知基因型抗原特性的比较。
Generation of hepatitis E virus-like particles of two new genotypes G5 and G6 and comparison of antigenic properties with those of known genotypes.
作者信息
Li Tian-Cheng, Kataoka Michiyo, Takahashi Kazuaki, Yoshizaki Sayaka, Kato Takanobu, Ishii Koji, Takeda Naokazu, Mishiro Shunji, Wakita Takaji
机构信息
Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan.
Department of Pathology, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan.
出版信息
Vet Microbiol. 2015 Jul 9;178(1-2):150-7. doi: 10.1016/j.vetmic.2015.04.020. Epub 2015 Apr 24.
In addition to the four major genotypes (G1 through G4) known for human hepatitis E virus (HEV), two new genotypes (G5 and G6) were suggested, based on unique viral nucleotide sequences derived from wild boars in Japan. It has been unknown whether the virus of these new genotypes can cause hepatitis in humans; neither G5 nor G6 HEV has been found in patients to date. To study the antigenic properties of G5 and G6 HEV, we expressed N-terminus-truncated HEV ORF2 protein by a recombinant baculovirus system, and we obtained virus-like particles (VLPs) for both G5 and G6. The VLPs showed antigenic cross-reactivity against G1, G3 and G4 HEV more strongly than against ferret or rat HEV. Moreover, both anti-G5 and anti-G6 VLPs antibodies could neutralize G3 HEV's ability to infect PLC/PRF/5 cells, suggesting that G5 and G6 HEV have the same serotype as human HEV.
除了已知的人类戊型肝炎病毒（HEV）的四种主要基因型（G1至G4）外，基于源自日本野猪的独特病毒核苷酸序列，还提出了两种新的基因型（G5和G6）。这些新基因型的病毒是否会导致人类肝炎尚不清楚；迄今为止，患者中尚未发现G5或G6型戊型肝炎病毒。为了研究G5和G6型戊型肝炎病毒的抗原特性，我们通过重组杆状病毒系统表达了N端截短的HEV ORF2蛋白，并获得了G5和G6型的病毒样颗粒（VLP）。与雪貂或大鼠戊型肝炎病毒相比，这些病毒样颗粒对G1、G3和G4型戊型肝炎病毒表现出更强的抗原交叉反应性。此外，抗G5和抗G6病毒样颗粒抗体都能中和G3型戊型肝炎病毒感染PLC/PRF/5细胞的能力，这表明G5和G6型戊型肝炎病毒与人类戊型肝炎病毒具有相同的血清型。
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