[Interaction of thrombocytes with immobilized collagen in the vascular wall (a model for step-by-step study of formation of parietal thrombi)].

Scanning electron microscopy was used to examine the interaction of platelets with types I, III, IV, and V human collagens (CI, CIII, CIV, and CV substrates) immobilized on the surface of cultural plates. Basic differences were found in the activity of collagen substrates against platelets. On the CV substrate, virtually all the adhesive platelets were in a non-spreading++ state, i.e. at the stage of initial attachment to the spread. The CIV substrate showed initial attachment, spreading, and attachment of platelets from the suspension to the spread platelets. In addition to the processes occurring at the CIV substrate, the CI and CIII substrates produced multilayer platelet aggregates on the strata of spreading platelets (thromboid aggregates). The surfaces covered with various types of collagens are an adequate model for quantitative step-by-step study of the formation of parietal thrombocytic thrombi and of mechanisms of this process control.