类风湿关节炎滑膜组织和血浆中血小板反应蛋白1表达上调：转化生长因子-β1对成纤维样滑膜细胞的作用
Upregulation of Thrombospondin 1 Expression in Synovial Tissues and Plasma of Rheumatoid Arthritis: Role of Transforming Growth Factor-β1 toward Fibroblast-like Synovial Cells.
作者信息
Suzuki Takahisa, Iwamoto Naoki, Yamasaki Satoshi, Nishino Ayako, Nakashima Yoshikazu, Horai Yoshiro, Kawashiri Shin-Ya, Ichinose Kunihiro, Arima Kazuhiko, Tamai Mami, Nakamura Hideki, Origuchi Tomoki, Miyamoto Chikara, Osaki Makoto, Ohyama Kaname, Kuroda Naotaka, Kawakami Atsushi
机构信息
From the Department of Immunology and Rheumatology, Department of Public Health, Department of Health Sciences, Department of Orthopedic Surgery, and Department of Environmental and Pharmaceutical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki; and Department of Clinical Immunology and Rheumatology, Hiroshima University, Hiroshima, Japan.T. Suzuki, MD; N. Iwamoto, MD, PhD, Assistant Professor, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences; S. Yamasaki, MD, PhD, Department of Clinical Immunology and Rheumatology, Hiroshima University; A. Nishino, MD; Y. Nakashima, MD; Y. Horai, MD, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences; S.Y. Kawashiri, MD, PhD, Department of Immunology and Rheumatology, and Department of Public Health, Nagasaki University Graduate School of Biomedical Sciences; K. Ichinose, MD, PhD, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences; K. Arima, MD, PhD, Department of Public Health, Nagasaki University Graduate School of Biomedical Sciences; M. Tamai, MD, PhD; H. Nakamura, MD, PhD, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences; T. Origuchi, MD, PhD, Professor, Department of Health Sciences, Nagasaki University Graduate School of Biomedical Sciences; C. Miyamoto, MD, PhD; M. Osaki, MD, PhD, Professor, Department of Orthopedic Surgery, Nagasaki University Graduate School of Biomedical Sciences; K. Ohyama, PhD; N. Kuroda, PhD, Professor, Department of Environmental and Pharmaceutical Sciences, Nagasaki University Graduate School of Biomedical Sciences; A. Kawakami, MD, PhD, Professor, Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences.
出版信息
J Rheumatol. 2015 Jun;42(6):943-7. doi: 10.3899/jrheum.141292. Epub 2015 May 1.
OBJECTIVE
To investigate the role of thrombospondin 1 (TSP-1) in RA.
METHODS
Expression of TSP-1 in synovial tissues was determined by immunohistochemistry. Expression of TSP-1 in rheumatoid fibroblast-like synovial cells (FLS) was investigated by quantitative real-time PCR and ELISA. Correlations among the plasma TSP-1 and other variables in patients with RA were examined.
RESULTS
Expression of TSP-1 was increased in rheumatoid synovial tissues. Transforming growth factor-β1 (TGF-β1) clearly increased TSP-1 expression in FLS on both mRNA and protein levels. Changes in plasma TSP-1 were associated with those in 28-joint Disease Activity Score-erythrocyte sedimentation rate and plasma TGF-β1.
CONCLUSION
TSP-1 might be critically involved in the disease process of RA through the TGF-β1/TSP-1 axis.
目的
研究血小板反应蛋白1（TSP-1）在类风湿关节炎（RA）中的作用。
方法
采用免疫组织化学法检测滑膜组织中TSP-1的表达。通过定量实时聚合酶链反应和酶联免疫吸附测定法研究类风湿成纤维样滑膜细胞（FLS）中TSP-1的表达。检测RA患者血浆TSP-1与其他变量之间的相关性。
结果
类风湿滑膜组织中TSP-1的表达增加。转化生长因子-β1（TGF-β1）在mRNA和蛋白质水平上均明显增加FLS中TSP-1的表达。血浆TSP-1的变化与28关节疾病活动评分-红细胞沉降率和血浆TGF-β1的变化相关。
结论
TSP-1可能通过TGF-β1/TSP-1轴在RA的疾病过程中起关键作用。
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