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基于iTRAQ-PRM的定量蛋白质组学揭示了痛风的新特征。

Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for gout.

作者信息

Chen Guangqi, Cheng Jiafen, Yu Hanjie, Huang Xiao, Bao Hui, Qin Ling, Wang Ling, Song Yaxiang, Liu Xinying, Peng Ai

机构信息

Center for Nephrology and Clinical Metabolomics and Division of Nephrology and Rheumatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, PR China.

出版信息

Proteome Sci. 2021 Oct 11;19(1):12. doi: 10.1186/s12953-021-00180-0.

DOI:10.1186/s12953-021-00180-0
PMID:34635120
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8507311/
Abstract

BACKGROUND

Gout is a common and complex form of immunoreactive arthritis based on hyperuricemia, while the symptoms would turn to remission or even got worse. So, it is hard to early identify whether an asymptomatic hyperuricemia (AHU) patient will be susceptible to get acute gout attack and it is also hard to predict the process of gout remission to flare. Here, we report that the plasma proteins profile can distinguish among acute gout (AG), remission of gout (RG), AHU patients, and healthy controls.

METHODS

We established an isobaric tags for relative and absolute quantification (iTRAQ) and parallel reaction monitoring (PRM) based method to measure the plasma proteins for AG group (n = 8), RG group (n = 7), AHU group (n = 7) and healthy controls (n = 8).

RESULTS

Eleven differentially expressed proteins such as Histone H2A, Histone H2B, Thrombospondin-1 (THBS1), Myeloperoxidase (MPO), Complement C2, Complement component C8 beta chain (C8B), Alpha-1-acid glycoprotein 1 (ORM1), Inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), Carbonic anhydrase 1 (CA1), Serum albumin (ALB) and Multimerin-1 (MMRN1) were identified. Histone H2A, Histone H2B and THBS1 might be the strongest influential regulator to maintain the balance and stability of the gout process. The complement and coagulation cascades is one of the main functional pathways in the mechanism of gout process.

CONCLUSIONS

Histone H2A, Histone H2B and THBS1 are potential candidate genes for novel biomarkers in discriminating gout attack from AHU or RG, providing new theoretical insights for the prognosis, treatment, and management of gout process.

TRIAL REGISTRATION

This study is not a clinical trial.

摘要

背景

痛风是一种基于高尿酸血症的常见且复杂的免疫反应性关节炎，其症状会缓解甚至加重。因此，很难早期识别无症状高尿酸血症（AHU）患者是否易患急性痛风发作，也难以预测痛风缓解至发作的过程。在此，我们报告血浆蛋白质谱可区分急性痛风（AG）、痛风缓解期（RG）、AHU患者和健康对照。

方法

我们建立了一种基于等压标签相对和绝对定量（iTRAQ）和平行反应监测（PRM）的方法，以测量AG组（n = 8）、RG组（n = 7）、AHU组（n = 7）和健康对照组（n = 8）的血浆蛋白质。

结果

鉴定出11种差异表达蛋白，如组蛋白H2A、组蛋白H2B、血小板反应蛋白-1（THBS1）、髓过氧化物酶（MPO）、补体C2、补体成分C8β链（C8B）、α-1-酸性糖蛋白1（ORM1）、间α-胰蛋白酶抑制剂重链H4（ITIH4）、碳酸酐酶1（CA1）、血清白蛋白（ALB）和多聚蛋白-1（MMRN1）。组蛋白H2A、组蛋白H2B和THBS1可能是维持痛风过程平衡和稳定性的最强影响调节因子。补体和凝血级联反应是痛风发病机制中的主要功能途径之一。

结论

组蛋白H2A、组蛋白H2B和THBS1是区分痛风发作与AHU或RG的新型生物标志物的潜在候选基因，为痛风的预后、治疗和管理提供了新的理论见解。

试验注册

本研究不是临床试验。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2423/8507311/718023e93f07/12953_2021_180_Fig6_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2423/8507311/718023e93f07/12953_2021_180_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2423/8507311/340ede2f9783/12953_2021_180_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2423/8507311/eb8819ccfdd9/12953_2021_180_Fig2_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2423/8507311/718023e93f07/12953_2021_180_Fig6_HTML.jpg

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