Juan Chi-Chang, Chen Kuo-Hu, Wang Peng-Hui, Hwang Jiann-Loung, Seow Kok-Min
Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan; Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan; Department of Medical Research and Education, Taipei City Hospital, Taipei, Taiwan.
Department of Obstetrics and Gynecology, Taipei Tzu-Chi Hospital, The Buddhist Tzu-Chi Medical Foundation, Taipei, Taiwan; School of Medicine, Buddhist Tzu-Chi University, Hualien, Taiwan.
Fertil Steril. 2015 Jul;104(1):200-6. doi: 10.1016/j.fertnstert.2015.03.027. Epub 2015 Apr 29.
To assess the levels of endocannabinoids and cannabinoid receptors (CB) 1 and 2 in women with polycystic ovary syndrome (PCOS).
Case-control study.
University teaching hospital.
PATIENT(S): In total, 20 women with PCOS and 20 healthy women in a control group, who were matched for body mass index and age, were enrolled in this study.
INTERVENTION(S): The homeostasis model index was used to assess insulin resistance.
MAIN OUTCOME MEASURE(S): Omental adipose tissue and human peripheral blood mononuclear cells (PBMCs) from PCOS and the controls were analyzed using real-time polymerase chain reactions for the expressions of CB1 and CB2. The levels of endocannabinoids were analyzed using high-performance liquid chromatography.
RESULT(S): The levels of anandamide and 2-arachidonoylglycerol, and the expression of CB1 and CB2 mRNA (messenger ribonucleic acid) in the PBMCs were significantly higher in the women with PCOS than in the women serving as controls. We found that expression of CB1, but not CB2, in adipose tissue was significantly higher in the women with, vs. without, PCOS. The expressions of CB1 mRNA and endocannabinoids showed a significant positive correlation with 2-hour glucose and insulin levels 2 hours after glucose loading in the PBMCs and adipose tissue.
CONCLUSION(S): Activation of endocannabinoids and overexpression of cannabinoid receptors, especially CB1, may be associated with insulin resistance in women with PCOS.
评估多囊卵巢综合征（PCOS）女性体内内源性大麻素以及大麻素受体1（CB1）和2（CB2）的水平。
病例对照研究。
大学教学医院。
本研究共纳入20例PCOS女性和20例健康女性作为对照组，两组在体重指数和年龄方面相匹配。
采用稳态模型指数评估胰岛素抵抗。
运用实时聚合酶链反应分析PCOS患者和对照组的网膜脂肪组织及人外周血单个核细胞（PBMCs）中CB1和CB2的表达情况。采用高效液相色谱法分析内源性大麻素水平。
PCOS女性的花生四烯乙醇胺和2-花生四烯酸甘油水平以及PBMCs中CB1和CB2信使核糖核酸（mRNA）的表达显著高于对照组女性。我们发现，PCOS女性脂肪组织中CB1而非CB2的表达显著高于非PCOS女性。PBMCs和脂肪组织中，CB1 mRNA和内源性大麻素的表达与葡萄糖负荷后2小时血糖和胰岛素水平呈显著正相关。
内源性大麻素的激活和大麻素受体尤其是CB1的过表达可能与PCOS女性的胰岛素抵抗有关。
