Bawden S J, Stephenson M C, Ciampi E, Hunter K, Marciani L, Macdonald I A, Aithal G P, Morris P G, Gowland P A
Sir Peter Manfield Imaging Centre, University of Nottingham, Nottingham, UK.
Sir Peter Manfield Imaging Centre, University of Nottingham, Nottingham, UK.
Clin Nutr. 2016 Jun;35(3):645-9. doi: 10.1016/j.clnu.2015.04.001. Epub 2015 Apr 14.
Impaired homeostasis of hepatic ATP has been associated with NAFLD. An intravenous fructose infusion has been shown to be an effective challenge to monitor the depletion and subsequent recovery of hepatic ATP reserves using (31)P MRS.
The purpose of this study was to evaluate the effects of an oral rather than intravenous fructose challenge on hepatic ATP reserves in healthy subjects.
Self-reported healthy males were recruited. Following an overnight fast, baseline liver glycogen and lipid levels were measured using Magnetic Resonance Spectroscopy (MRS). Immediately after consuming a 500 ml 75 g fructose drink (1275 kJ) subjects were scanned continuously for 90 min to acquire dynamic (31)P MRS measurements of liver ATP reserves.
A significant effect on ATP reserves was observed across the time course (P < 0.05). Mean ATP levels reached a minimum at 50 min which was markedly lower than baseline (80 ± 17% baseline, P < 0.05). Subsequently, mean values tended to rise but did not reach statistical significance above minimum. The time to minimum ATP levels across subjects was negatively correlated with BMI (R(2) = 0.74, P < 0.005). Rates of ATP recovery were not significantly correlated with BMI or liver fat levels, but were negatively correlated with baseline glycogen levels (R(2) = 0.7, P < 0.05).
Depletion of ATP reserves can be measured non-invasively following an oral fructose challenge using (31)P MRS. BMI is the best predictor of postprandial ATP homeostasis following fructose consumption.
肝脏ATP内稳态受损与非酒精性脂肪性肝病（NAFLD）相关。静脉输注果糖已被证明是一种有效的激发试验，可使用磷-31磁共振波谱（³¹P MRS）监测肝脏ATP储备的消耗及随后的恢复情况。
本研究旨在评估口服而非静脉输注果糖激发试验对健康受试者肝脏ATP储备的影响。
招募自我报告健康的男性。经过一夜禁食后，使用磁共振波谱（MRS）测量基线肝糖原和脂质水平。在饮用500毫升含75克果糖的饮料（1275千焦）后，立即对受试者进行90分钟的连续扫描，以获取肝脏ATP储备的动态磷-31磁共振波谱测量值。
在整个时间过程中观察到ATP储备有显著影响（P < 0.05）。平均ATP水平在50分钟时达到最低，明显低于基线水平（为基线的80 ± 17%，P < 0.05）。随后，平均值趋于上升，但未达到显著高于最低值的统计学意义。所有受试者达到最低ATP水平的时间与体重指数（BMI）呈负相关（R² = 0.74，P < 0.005）。ATP恢复率与BMI或肝脏脂肪水平无显著相关性，但与基线糖原水平呈负相关（R² = 0.7，P < 0.05）。
口服果糖激发试验后，可使用磷-31磁共振波谱非侵入性地测量ATP储备的消耗情况。BMI是摄入果糖后餐后ATP内稳态的最佳预测指标。
