Ngieng Ngui Sing, Zulkharnain Azham, Roslan Hairul Azman, Husaini Ahmad
Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
ISRN Biotechnol. 2013 Jul 22;2013:260730. doi: 10.5402/2013/260730. eCollection 2013.
A total of twenty endophytic fungi successfully isolated from Melastoma malabathricum (Senduduk) were examined for their ability to decolourise azo dyes: Congo red, Orange G, and Methyl red and an anthraquinone dye, Remazol Brilliant Blue R. Initial screening on the glucose minimal media agar plates amended with 200 mg L(-1) of each respective dye showed that only isolate MS8 was able to decolourise all of the four dyes. The isolate decolourised completely both the RBBR and Orange G in the agar medium within 8 days. Further quantitative analysis of the dye decolourisation by isolate MS8 in aqueous minimal medium showed that isolate MS8 was able to decolourise all the tested dyes at varying levels. Dye decolourisation by the isolate MS8 was determined to be 97% for RBBR, 33% for Orange G, 48% for Congo red, and 56% for Methyl red, respectively, within a period of 16 days. Molecular identification of the fungal isolate MS8 using primer ITS1 and ITS4 showed that isolate MS8 shared 99% sequence similarity with Marasmius cladophyllus, a Basidiomycete. The ability to decolourise different types of dyes by isolate MS8 thus suggested a possible application of this fungus in the decolourisation of dyestuff effluents.
从毛稔（Senduduk）中成功分离出20种内生真菌，检测它们对偶氮染料（刚果红、橙黄G和甲基红）以及蒽醌染料（雷马素亮蓝R）的脱色能力。在添加了200 mg L⁻¹各染料的葡萄糖基本培养基琼脂平板上进行初步筛选，结果表明只有菌株MS8能够使所有四种染料脱色。该菌株在8天内使琼脂培养基中的RBBR和橙黄G完全脱色。对菌株MS8在水基本培养基中染料脱色的进一步定量分析表明，菌株MS8能够在不同程度上使所有测试染料脱色。在16天内，菌株MS8对RBBR、橙黄G、刚果红和甲基红的染料脱色率分别为97%、33%、48%和56%。使用引物ITS1和ITS4对真菌菌株MS8进行分子鉴定，结果表明菌株MS8与担子菌纲的枝状小皮伞序列相似性为99%。因此，菌株MS8对不同类型染料的脱色能力表明这种真菌在染料废水脱色方面可能具有应用价值。
