Salihu Aliyu, Bala Muntari, Bala Shuaibu M
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
Department of Biochemistry, Bayero University, Kano, Nigeria.
ISRN Biotechnol. 2013 Feb 13;2013:718352. doi: 10.5402/2013/718352. eCollection 2013.
Plackett-Burman design was used to efficiently select important medium components affecting the lipase production by Aspergillus niger using shea butter cake as the main substrate. Out of the eleven medium components screened, six comprising of sucrose, (NH4)2SO4, Na2HPO4, MgSO4, Tween-80, and olive oil were found to contribute positively to the overall lipase production with a maximum production of 3.35 U/g. Influence of tween-80 on lipase production was investigated, and 1.0% (v/w) of tween-80 resulted in maximum lipase production of 6.10 U/g. Thus, the statistical approach employed in this study allows for rapid identification of important medium parameters affecting the lipase production, and further statistical optimization of medium and process parameters can be explored using response surface methodology.
采用Plackett-Burman设计法，以乳木果饼为主要底物，高效筛选影响黑曲霉产脂肪酶的重要培养基成分。在所筛选的11种培养基成分中，发现蔗糖、硫酸铵、磷酸氢二钠、硫酸镁、吐温80和橄榄油这6种成分对脂肪酶的总体产量有正向贡献，最高产量为3.35 U/g。研究了吐温80对脂肪酶产量的影响，1.0%（v/w）的吐温80可使脂肪酶最高产量达到6.10 U/g。因此，本研究采用的统计方法能够快速识别影响脂肪酶产量的重要培养基参数，并且可以使用响应面法进一步对培养基和工艺参数进行统计优化。
