Himalayan School of Biosciences, Swami Rama Himalayan University, Swami Rama Nagar, Jolly Grant, Dehradun, Uttarakhand, 248016, India.
Department of Life Sciences, Food Technology, Graphic Era Deemed to be University, Dehradun, 248002, India.
Sci Rep. 2022 Jul 25;12(1):12661. doi: 10.1038/s41598-022-16854-4.
One of the key enzymes utilized in the food industry is pullulanase. But its major drawbacks are its low yield and high production costs. In this regard, the current research aims to screen agro-waste substrates for optimal pullulanase production in solid-state fermentation. Of various agro-wastes used as a substrate, the maximum enzymic activity (9.74 U/gds) was observed in a medium based on 5 g of green gram husk and incubated for 3 days at 30 °C. The effects of 16 different nutrients on the yield of pullulanase production were studied using the Plackett-Burman experimental design. The incorporation of FeSO, MnSO, and MgSO into the pullulanase production medium significantly increased the yield and showed a 5.7-fold increase (56.25 U/gds) in comparison with the unoptimized media. The Box-Behnken experimental design was used to study the effect of interactions between Fe, Mg, and Mn on the production of pullulanase. Box-Behnken showed a 1.1-fold increase (62.1 U/gds) in pullulanase production. The total increase in yield after all optimization was 6.37-fold. The present study reports for the first time the applicability of green gram husk as a potent substrate for pullulanase production by Penicillium viridicatum.
在食品工业中，一种关键的酶是普鲁兰酶。但它的主要缺点是产量低、生产成本高。在这方面，目前的研究旨在筛选农业废料作为固态发酵中生产普鲁兰酶的最佳底物。在所使用的各种农业废料中，在基于 5 克绿豆皮的培养基中观察到最大的酶活性（9.74 U/gds），并在 30°C 下培养 3 天。使用 Plackett-Burman 实验设计研究了 16 种不同的营养物质对普鲁兰酶产量的影响。在普鲁兰酶生产培养基中加入 FeSO、MnSO 和 MgSO 显著提高了产量，与未优化的培养基相比，产量增加了 5.7 倍（56.25 U/gds）。使用 Box-Behnken 实验设计研究了 Fe、Mg 和 Mn 之间相互作用对普鲁兰酶生产的影响。Box-Behnken 显示普鲁兰酶产量增加了 1.1 倍（62.1 U/gds）。所有优化后的总产量增加了 6.37 倍。本研究首次报道了绿豆皮作为米曲霉生产普鲁兰酶的有效底物的适用性。
