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从土壤分离细菌中纤维素酶产量的优化。

Optimization of cellulase production from bacteria isolated from soil.

作者信息

Sethi Sonia, Datta Aparna, Gupta B Lal, Gupta Saksham

机构信息

Department of Biotechnology, Dr. B. Lal Institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur 302017, India.

出版信息

ISRN Biotechnol. 2013 Feb 19;2013:985685. doi: 10.5402/2013/985685. eCollection 2013.

DOI:10.5402/2013/985685
PMID:25937986
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4393041/
Abstract

Cellulase-producing bacteria were isolated from soil and identified as Pseudomonas fluorescens, Bacillus subtilIs, E. coli, and Serratia marcescens. Optimization of the fermentation medium for maximum cellulase production was carried out. The culture conditions like pH, temperature, carbon sources, and nitrogen sources were optimized. The optimum conditions found for cellulase production were 40°C at pH 10 with glucose as carbon source and ammonium sulphate as nitrogen source, and coconut cake stimulates the production of cellulase. Among bacteria, Pseudomonas fluorescens is the best cellulase producer among the four followed by Bacillus subtilis, E. coli, and Serratia marscens.

摘要

从土壤中分离出了产纤维素酶的细菌，并鉴定为荧光假单胞菌、枯草芽孢杆菌、大肠杆菌和粘质沙雷氏菌。对发酵培养基进行了优化以实现最大量的纤维素酶生产。对pH值、温度、碳源和氮源等培养条件进行了优化。发现纤维素酶生产的最佳条件是在pH值为10、温度为40°C时，以葡萄糖作为碳源，硫酸铵作为氮源，并且椰子饼能刺激纤维素酶的产生。在这些细菌中，荧光假单胞菌是这四种细菌中最佳的纤维素酶生产者，其次是枯草芽孢杆菌、大肠杆菌和粘质沙雷氏菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/21ede2e8f260/ISRN.BIOTECHNOLOGY2013-985685.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/a18a8ff86e09/ISRN.BIOTECHNOLOGY2013-985685.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/6828325d61b4/ISRN.BIOTECHNOLOGY2013-985685.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/69cc07685460/ISRN.BIOTECHNOLOGY2013-985685.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/8ef49e77987a/ISRN.BIOTECHNOLOGY2013-985685.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/4434a777ab24/ISRN.BIOTECHNOLOGY2013-985685.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/afb59558d87e/ISRN.BIOTECHNOLOGY2013-985685.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/2b973f434612/ISRN.BIOTECHNOLOGY2013-985685.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/ae1e4e14f2ed/ISRN.BIOTECHNOLOGY2013-985685.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/6b007cde584d/ISRN.BIOTECHNOLOGY2013-985685.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/21ede2e8f260/ISRN.BIOTECHNOLOGY2013-985685.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/a18a8ff86e09/ISRN.BIOTECHNOLOGY2013-985685.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/6828325d61b4/ISRN.BIOTECHNOLOGY2013-985685.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/69cc07685460/ISRN.BIOTECHNOLOGY2013-985685.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/8ef49e77987a/ISRN.BIOTECHNOLOGY2013-985685.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/4434a777ab24/ISRN.BIOTECHNOLOGY2013-985685.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/afb59558d87e/ISRN.BIOTECHNOLOGY2013-985685.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/2b973f434612/ISRN.BIOTECHNOLOGY2013-985685.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/ae1e4e14f2ed/ISRN.BIOTECHNOLOGY2013-985685.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/6b007cde584d/ISRN.BIOTECHNOLOGY2013-985685.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7442/4393041/21ede2e8f260/ISRN.BIOTECHNOLOGY2013-985685.010.jpg

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