具有聚集诱导发光特性的光稳定荧光线粒体特异性生物探针实时监测细胞的线粒体自噬过程
Real-time monitoring of the mitophagy process by a photostable fluorescent mitochondrion-specific bioprobe with AIE characteristics.
机构信息
Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
出版信息
Chem Commun (Camb). 2015 May 28;51(43):9022-5. doi: 10.1039/c5cc02486b.
PMID:25939788
Abstract
An isothiocyanate-functionalized tetraphenylethene is synthesized and used as a fluorescent bioprobe for mitochondrion imaging with high specificity and photostability. The covalent conjugation of the bioprobe to mitochondrial proteins endows it with high resistance to microenvironmental changes, enabling it for real-time monitoring of mitophagy.
摘要
合成了一种异硫氰酸酯功能化的四苯乙烯，并将其用作荧光生物探针，用于线粒体成像，具有高特异性和光稳定性。生物探针与线粒体蛋白的共价连接赋予其对微环境变化的高抵抗力，使其能够实时监测噬线粒体。
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