线粒体特异性荧光开启型活细胞生物探针及其精心设计的光激活机制
Mitochondrion-Specific Live-Cell Bioprobe Operated in a Fluorescence Turn-On Manner and a Well-Designed Photoactivatable Mechanism.
机构信息
HKUST-Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-Tech Park, Nanshan, Shenzhen, 518057, China.
Departments of Chemistry, Physics, and Division of Life Science, Institute of Molecular Functional Materials and Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
出版信息
Adv Mater. 2015 Nov 25;27(44):7093-100. doi: 10.1002/adma.201503751. Epub 2015 Oct 7.
A novel mitochondrion-specific live-cell fluorescence turn-on photoactivatable bioprobe, named o-TPP3M, is designed and readily prepared, operating in a new photoactivatable mechanism based on a twisted intramolecular charge-transfer effect, the restriction of intramolecular rotations, and photocyclodehydrogenation. This bioprobe exhibits unique photoactivation behavior, a large signal-to-background ratio, and no cytotoxicity to living cells, providing a promising candidate for varied bioresearch.
一种新型的线粒体特异性活细胞荧光开启光致活性生物探针，命名为 o-TPP3M，被设计并易于制备，其工作原理基于扭曲的分子内电荷转移效应、分子内旋转的限制和光环脱氢作用，属于一种新的光致活性机制。这种生物探针表现出独特的光激活行为、较大的信号背景比以及对活细胞无细胞毒性，为各种生物研究提供了有前途的候选物。
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