利用循环逆流色谱法从决明子种子中分离蒽醌类化合物。

Separation of anthraquinone compounds from the seed of Cassia obtusifolia L. using recycling counter-current chromatography.

作者信息

Yang Jianhong, Ye Haoyu, Lai Huijun, Li Shucai, He Shichao, Zhong Shijie, Chen Lijuan, Peng Aihua

机构信息

State Key Laboratory of Biotherapy, West China Hospital and College of Pharmacy, Sichuan University, Chengdu, Sichuan, P. R. China; State Key Laboratory Breeding Base of Systematic Research Development of Utilization of Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, P. R. China.

出版信息

J Sep Sci. 2012 Jan;35(2):256-62. doi: 10.1002/jssc.201100535.

DOI:10.1002/jssc.201100535

PMID:25939902

Abstract

Recycling counter-current chromatography (CCC) together with step-gradient CCC and medium-pressure liquid chromatography (MPLC) was employed to separate nine anthraquinone compounds from Cassia obtusifolia L. in this study. The results showed that recycling CCC is a powerful tool for compounds that are difficult to separate with common elution mode. CCC was the better option for crude material while MPLC had advantage for the final tuning. The combination of recycling CCC and MPLC could simplify the method exploring process in the separation process. The structures of these compounds were identified according to their mass spectra, by (1)H-NMR and compared with standard compounds.

摘要

本研究采用循环逆流色谱（CCC）结合分步梯度CCC和中压液相色谱（MPLC）从决明子中分离出9种蒽醌类化合物。结果表明，循环CCC是分离难以用常规洗脱模式分离的化合物的有力工具。CCC对于粗品是更好的选择，而MPLC在最终纯化方面具有优势。循环CCC和MPLC的结合可以简化分离过程中的方法探索过程。根据这些化合物的质谱、（1）H-NMR并与标准化合物进行比较来鉴定其结构。