Guzmán R, Campos C, Yuguero R, Masegù C, Gil P, Moragón Ángela Casado
Departamento de Medicina Celular y Molecular, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu, 9, 28040 Madrid, Spain.
Unidad de Memoria Servicio de Geriatría, Hospital Clínico San Carlos, Profesor Martín Lagos s/n, 28040 Madrid, Spain.
Life Sci. 2015 Jul 1;132:61-7. doi: 10.1016/j.lfs.2015.04.006. Epub 2015 May 2.
One of the main features of sulfurous water (SW) is the presence of hydrogen sulfide (H2S), which confers its antioxidant activity. Since oxidative stress plays an important role in Alzheimer's disease (AD) we hypothesize that SW could have a protective effect in these patients.
A therapeutic in vitro approach of SW was performed in peripheral blood mononuclear cells (PBMCs) of AD patients and in age-matched healthy non-demented controls using one modification of the comet assay (to measure oxidative DNA damage) and the MTT assay (as an indicator of cell viability). Hydrogen peroxide and homocysteine were used to induce oxidative DNA damage, and vitamin C, Trolox and N-acetyl-cysteine were selected as antioxidants of reference to compare SW treatment results.
SW did not increase per se the oxidative DNA damage of PBMC. Furthermore, SW protected them against enhanced oxidative stress in AD and control populations after pro-oxidant stimuli, with similar results to those observed when using the antioxidants of reference. Nevertheless, SW was the only treatment that could avoid the loss of viability of PBMC for all pro-oxidant stimuli in both populations, suggesting that H2S could confer to SW a more antioxidant capacity than other known antioxidants.
The protective effect of SW was proved for the first time not only in DNA stability but also in cell viability preservation in AD, indicating that further research in other in vitro and in vivo models could lead to include SW as a possible therapy for AD.
含硫水（SW）的主要特征之一是存在硫化氢（H2S），这赋予了其抗氧化活性。由于氧化应激在阿尔茨海默病（AD）中起重要作用，我们推测SW可能对这些患者具有保护作用。
采用彗星试验的一种改良方法（用于测量氧化性DNA损伤）和MTT试验（作为细胞活力指标），对AD患者的外周血单核细胞（PBMC）和年龄匹配的健康非痴呆对照进行SW的体外治疗研究。用过氧化氢和同型半胱氨酸诱导氧化性DNA损伤，并选择维生素C、Trolox和N-乙酰半胱氨酸作为参考抗氧化剂来比较SW的治疗效果。
SW本身不会增加PBMC的氧化性DNA损伤。此外，在促氧化剂刺激后，SW可保护AD患者和对照人群的PBMC免受增强的氧化应激影响，其结果与使用参考抗氧化剂时观察到的结果相似。然而，SW是唯一能避免两种人群中所有促氧化剂刺激导致PBMC活力丧失的治疗方法，这表明H2S可能赋予SW比其他已知抗氧化剂更强的抗氧化能力。
首次证明了SW不仅对AD患者的DNA稳定性有保护作用，而且对细胞活力的保存也有保护作用，这表明在其他体外和体内模型中进一步研究可能会使SW成为AD的一种可能治疗方法。
