Department of Biotechnology, Alagappa University, Karaikudi 630 003, Tamil Nadu, India.
Chemosphere. 2011 Aug;84(7):888-93. doi: 10.1016/j.chemosphere.2011.06.017. Epub 2011 Jul 7.
The protective effect of hydroxytyrosol (HT), a strong antioxidant compound from extra virgin olive oil, against TCDD induced toxicity was investigated in human peripheral blood mononuclear cells (PBMC). PBMC (1 × 10(6)cellsmL(-1)) were divided into four groups and were incubated in a CO(2) incubator (5% CO(2)) for 12h with vehicle, TCDD (10 nM), TCDD+HT (10 nM+100 μM) and HT alone (100 μM) respectively. To clarify the role of HT against TCDD induced cytotoxicity, oxidative stress and the levels of antioxidant enzymes were assessed. Incubation of PBMC with TCDD significantly decreased cell viability, catalase (CAT) and glutathione peroxidase (GPx) and increased the levels of superoxide dismutase (SOD), glutathione reductase (GR) and oxidative stress markers such as lipid peroxidation products (LPO), protein carbonyl content (PCC) and reactive oxygen species (ROS). Whereas, HT had an effective antioxidant property as observed by the increased cell viability, normalization of antioxidant enzymes and decreased levels of LPO, PCC and ROS in PBMC co-treated with HT and TCDD. Apoptosis detection and comet assay results shows that HT, by acting as an antioxidant, prevents the damage to DNA induced by TCDD. In addition light microscopic and histopathological observations revealed that the cells are apoptotic and degenerated during TCDD treatment, whereas cells showed intact morphology during co-treatment with HT. On the whole, the results reveal that HT exerts a promising antioxidant potential in protecting the PBMC against TCDD induced oxidative stress, which might be due to the presence of catechol moiety in its structure.
羟基酪醇（HT）是特级初榨橄榄油中的一种强抗氧化化合物，研究了其对 TCDD 诱导的毒性的保护作用，在人外周血单个核细胞（PBMC）中进行了研究。将 PBMC（1×10^6 个细胞/mL）分成四组，分别在 CO2 孵育箱（5% CO2）中孵育 12 小时，使用载体、TCDD（10 nM）、TCDD+HT（10 nM+100 μM）和 HT 单独（100 μM）孵育。为了阐明 HT 对 TCDD 诱导的细胞毒性、氧化应激和抗氧化酶水平的作用，评估了 HT 的作用。TCDD 孵育显著降低了 PBMC 的细胞活力、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的水平，增加了超氧化物歧化酶（SOD）、谷胱甘肽还原酶（GR）和氧化应激标志物如脂质过氧化产物（LPO）、蛋白质羰基含量（PCC）和活性氧（ROS）的水平。然而，HT 具有有效的抗氧化特性，如观察到的 PBMC 中细胞活力增加、抗氧化酶正常化以及 LPO、PCC 和 ROS 水平降低。凋亡检测和彗星试验结果表明，HT 通过作为抗氧化剂，防止 TCDD 诱导的 DNA 损伤。此外，光学显微镜和组织病理学观察显示，在 TCDD 处理期间细胞凋亡和退化，而在与 HT 共同处理期间细胞显示出完整的形态。总的来说，这些结果表明，HT 发挥了有希望的抗氧化潜力，可保护 PBMC 免受 TCDD 诱导的氧化应激，这可能是由于其结构中存在儿茶酚部分。
