Di Cesare Angela, Veronesi Fabrizia, Frangipane di Regalbono Antonio, De Liberato Claudio, Perrucci Stefania, Iorio Raffaella, Morganti Giulia, Marangi Marianna, Simonato Giulia, Traversa Donato
Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro 45, 64100 Teramo, Italy.
Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy.
Vet Parasitol. 2015 Jun 30;211(1-2):67-70. doi: 10.1016/j.vetpar.2015.04.016. Epub 2015 Apr 24.
Eucoleus böhmi (syn. Capillaria boehmi) is a trichuroid nematode affecting the epithelium of the nasal turbinates, frontal and paranasal sinuses of wild and domestic canids. Knowledge of the geographic distribution of nasal eucoleosis is fragmentary, despite the infection has been described from Europe and North America. Moreover, gaps exist in information available on the importance of the disease in canine clinical practice. The lack of knowledge on E. böhmi is likely due to limitations inherent to diagnostic methodologies. The aim of the present work was to assess a PCR-based assay instrumental to the amplification of a species-specific region of the mitochondrial DNA (mtDNA) gene encoding for the subunit 1 (cox1) gene of E. böhmi. Adult worms of E. böhmi from red foxes and dogs from Norway, Serbia and Italy and individual fecal samples from naturally infected dogs from Italy were included in the study. Stool samples from dogs negative for E. böhmi, but positive for other common parasites in both single and mixed infections, and adult stages of common dog parasites, were used to assess the specificity of this genetic assay. Using the panel of faecal samples, the assay showed a sensitivity of 85.14% and a specificity of 100%.
波氏真毛细线虫（学名：Eucoleus böhmi，异名：Capillaria boehmi）是一种毛首线虫，可感染野生和家养犬科动物的鼻甲、额窦和鼻窦上皮。尽管欧洲和北美均已报道过鼻真毛细线虫病感染情况，但关于该病地理分布的了解仍不完整。此外，关于该病在犬类临床实践中的重要性，现有信息也存在空白。对波氏真毛细线虫了解不足，可能是由于诊断方法本身存在局限性。本研究的目的是评估一种基于聚合酶链反应（PCR）的检测方法，该方法有助于扩增编码波氏真毛细线虫细胞色素c氧化酶亚基1（cox1）基因的线粒体DNA（mtDNA）基因的物种特异性区域。研究纳入了来自挪威、塞尔维亚和意大利的赤狐和犬体内的波氏真毛细线虫成虫，以及来自意大利自然感染犬的个体粪便样本。对波氏真毛细线虫检测呈阴性，但对其他常见寄生虫单感染或混合感染呈阳性的犬的粪便样本，以及常见犬寄生虫的成虫阶段样本，用于评估该基因检测方法的特异性。使用该粪便样本组，该检测方法的灵敏度为85.14%，特异性为100%。
