免疫造血干细胞增殖能力的丧失是由连续移植而非衰老所致。
Loss of proliferative capacity in immunohemopoietic stem cells caused by serial transplantation rather than aging.
作者信息
Harrison D E, Astle C M, Delaittre J A
出版信息
J Exp Med. 1978 May 1;147(5):1526-31. doi: 10.1084/jem.147.5.1526.
Abstract
Marrow stem cell lines from old donors and those from young controls gave equally rapid rates of colony growth on spleens of irradiated mice. Old and young stem cell lines competed equally well with chromosomally marked marrow stem cells from a young donor in producing cell types that are stimulated by bleeding; old cells competed 70% as well as young in producing cell types stimulated by phytohemagglutinin (PHA) in vitro. After a single serial transplantation, the rates of colony growth declined 1.5- to 2.5-fold, and the ability to compete declined 2- to 4-fold for bleeding-stimulated and 4- to 10-fold for PHA-stimulated cells. Thus, immediate stem cell proliferative capacities decline much more after one serial transplantation than after a lifetime of normal function.
摘要
来自老年供体的骨髓干细胞系和来自年轻对照的骨髓干细胞系，在受辐照小鼠脾脏上的集落生长速度相同。在产生受出血刺激的细胞类型方面，老年和年轻的干细胞系与来自年轻供体的染色体标记骨髓干细胞竞争能力相当；在体外产生受植物血凝素（PHA）刺激的细胞类型方面，老年细胞的竞争能力为年轻细胞的70%。单次连续移植后，集落生长速度下降了1.5至2.5倍，对于受出血刺激的细胞，竞争能力下降了2至4倍，对于受PHA刺激的细胞，竞争能力下降了4至10倍。因此，与正常功能一生之后相比，干细胞的即时增殖能力在一次连续移植后下降得更多。
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