Hoareau T B, Klopper A W, Dos Santos S M R, Oosthuizen C J, Bloomer P
Molecular Ecology and Evolution Programme, Department of Genetics, University of Pretoria, Private bag X20, Hatfield, 0028, South Africa.
J Fish Biol. 2015 May;86(5):1650-7. doi: 10.1111/jfb.12669.
The utility of 15 new and 17 previously published microsatellite markers was evaluated for species identification and stock delimitation in the deep-water hake Merluccius paradoxus and the shallow-water hake Merluccius capensis. A total of 14 microsatellites were polymorphic in M. paradoxus and 10 in M. capensis. Two markers could individually discriminate the species using Bayesian clustering methods and a statistical power analysis showed that the set of markers for each species is likely to detect subtle genetic differentiation (FST < 0·006) that will be valuable to delimit and characterize genetic stocks.
评估了15个新的和17个先前发表的微卫星标记在深水无须鳕（Merluccius paradoxus）和浅水无须鳕（Merluccius capensis）的物种鉴定和种群划分中的效用。总共有14个微卫星在深水无须鳕中具有多态性，10个在浅水无须鳕中具有多态性。使用贝叶斯聚类方法，两个标记能够单独区分这两个物种，并且统计功效分析表明，每个物种的标记集可能检测到细微的遗传分化（FST < 0·006），这对于界定和表征遗传种群将是有价值的。
