Perez Juliana, Garcia-Vazquez Eva
Departamento de Biologia Funcional, Universidad de Oviedo, C/ Julian Claveria s/n, 33006-Oviedo, Spain.
J Food Prot. 2004 Dec;67(12):2792-6. doi: 10.4315/0362-028x-67.12.2792.
This study describes the 5S rRNA gene as a simple and reliable one-step PCR-based genetic marker that allows unambiguous identification of Merluccius paradoxus, Merluccius senegalensis, Merluccius australis, Merluccius gayi, Merluccius bilinearis, Merluccius hubbsi, and Macruronus magellanicus. The marker is based on differences in length at the nontranscribed spacer within the 5S rRNA genes and is robust enough for identification of processed products, such as fish fingers and other precooked commercial hake. Further restriction fragment length polymorphism analysis at the mitochondrial cytochrome b gene can distinguish Merluccius merluccius and Merluccius capensis. The application of these markers for routine works such as surveys of quality and labeling of commercial hakes is suggested due to their accuracy, low price, and possible automation for large-scale surveys.
本研究将5S rRNA基因描述为一种简单可靠的基于一步PCR的遗传标记，可明确鉴定梅氏无须鳕、塞内加尔无须鳕、澳洲无须鳕、盖氏无须鳕、双线无须鳕、哈氏无须鳕和麦哲伦犬牙南极鱼。该标记基于5S rRNA基因非转录间隔区长度的差异，对于鱼条和其他预煮商业无须鳕等加工产品的鉴定也足够可靠。进一步对线粒体细胞色素b基因进行限制性片段长度多态性分析，可以区分欧洲无须鳕和南非无须鳕。鉴于这些标记具有准确性、低成本以及可用于大规模调查的自动化可能性，建议将其应用于商业无须鳕质量检测和标签标注等常规工作。
