Orphanou Charlotte-Maria, Walton-Williams Laura, Mountain Harry, Cassella John
Forensic & Crime Science, Staffordshire University, Science Centre, Leek Road, Stoke-on-Trent, Staffordshire ST4 2DF, United Kingdom.
Forensic Sci Int. 2015 Jul;252:e10-6. doi: 10.1016/j.forsciint.2015.04.020. Epub 2015 Apr 24.
Blood, saliva, semen and vaginal secretions are the main human body fluids encountered at crime scenes. Currently presumptive tests are routinely utilised to indicate the presence of body fluids, although these are often subject to false positives and limited to particular body fluids. Over the last decade more sensitive and specific body fluid identification methods have been explored, such as mRNA analysis and proteomics, although these are not yet appropriate for routine application. This research investigated the application of ATR FT-IR spectroscopy for the detection and discrimination of human blood, saliva, semen and vaginal secretions. The results demonstrated that ATR FT-IR spectroscopy can detect and distinguish between these body fluids based on the unique spectral pattern, combination of peaks and peak frequencies corresponding to the macromolecule groups common within biological material. Comparisons with known abundant proteins relevant to each body fluid were also analysed to enable specific peaks to be attributed to the relevant protein components, which further reinforced the discrimination and identification of each body fluid. Overall, this preliminary research has demonstrated the potential for ATR FT-IR spectroscopy to be utilised in the routine confirmatory screening of biological evidence due to its quick and robust application within forensic science.
血液、唾液、精液和阴道分泌物是犯罪现场常见的主要人体体液。目前，推定测试通常用于指示体液的存在，尽管这些测试常常会出现假阳性，并且仅限于特定的体液。在过去十年中，人们探索了更灵敏、更具特异性的体液识别方法，如mRNA分析和蛋白质组学，尽管这些方法尚未适用于常规应用。本研究调查了衰减全反射傅里叶变换红外光谱（ATR FT-IR光谱）在检测和区分人血、唾液、精液和阴道分泌物方面的应用。结果表明，ATR FT-IR光谱可以根据独特的光谱模式、峰的组合以及与生物材料中常见大分子基团相对应的峰频率来检测和区分这些体液。还分析了与每种体液相关的已知丰富蛋白质的比较结果，以使特定峰能够归因于相关蛋白质成分，这进一步加强了对每种体液的区分和识别。总体而言，这项初步研究表明，由于ATR FT-IR光谱在法医学中应用快速且可靠，因此有潜力用于生物证据的常规确证筛查。
