Król Paweł, Obmiński Zbigniew, Reich Adam, Czarny Wojciech, Cebulski Józef, Depciuch Joanna, Zamorski Michał, Stępień Katarzyna, Rydzik Łukasz
Department of Physical Education, Medical College of Rzeszow University, Rzeszow, Poland.
Department of Endocrinology, Institute of Sport-National Research Institute, Warsaw, Poland.
J Biophotonics. 2025 Sep;18(9):e202500173. doi: 10.1002/jbio.202500173. Epub 2025 Jun 22.
The literature lacks data on transient infrared spectral changes in the epidermis following physical exercise. This study tested the hypothesis that a single exercise session affects selected spectral bands (3270-1045 cm) in healthy individuals. Eight professional tennis players completed a 1.5-h moderate-intensity training session. Epidermal samples from the inner hand were collected before and after exercise, following cleaning with distilled water and 96% PA ethyl alcohol. Samples were analyzed using Fourier Transform Infrared Spectroscopy (FTIR). Absorbance values were recorded for 12 peaks. Significant correlations were observed for the 3270 cm (r = 0.976) and 1045 cm (r = 0.754) peaks. Notably, post-exercise increases were found at 1453 cm (lipids/proteins), 1078 cm (phospholipids), and 1045 cm (carbohydrates). No significant changes were observed for other peaks, though a general upward trend appeared. Inter-individual variability was high. FTIR may detect acute epidermal biochemical responses to exercise, especially in lipid- and phospholipid-related structures.
文献中缺乏关于体育锻炼后表皮瞬态红外光谱变化的数据。本研究检验了这样一个假设：单次锻炼会影响健康个体中选定的光谱带（3270 - 1045厘米）。八名职业网球运动员完成了一次1.5小时的中等强度训练课程。在锻炼前后，先用蒸馏水和96%的PA乙醇对手内侧进行清洁，然后采集表皮样本。使用傅里叶变换红外光谱（FTIR）对样本进行分析。记录了12个峰的吸光度值。在3270厘米（r = 0.976）和1045厘米（r = 0.754）的峰处观察到显著相关性。值得注意的是，锻炼后在1453厘米（脂质/蛋白质）、1078厘米（磷脂）和1045厘米（碳水化合物）处出现了增加。其他峰未观察到显著变化，不过呈现出总体上升趋势。个体间差异很大。FTIR可能检测到锻炼引起的急性表皮生化反应，尤其是在与脂质和磷脂相关的结构中。
