Balaji S, Kalaivani T, Rajasekaran C, Shalini M, Vinodhini S, Priyadharshini S Sunitha, Vidya A G
School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, 632 014, India.
Environ Monit Assess. 2015 Jun;187(6):325. doi: 10.1007/s10661-015-4440-7. Epub 2015 May 6.
The present study was carried out with the tannery effluent contaminated with heavy metals collected from Ambur industrial area to determine the phycoremediation potential of Arthrospira (Spirulina) platensis. Two different concentrations (50 and 100 %) of heavy metals containing tannery effluent treated with A. platensis were analysed for growth, absorption spectra, biochemical properties and antioxidant enzyme activity levels. The effluent treatments revealed dose-dependent decrease in the levels of A. platensis growth (65.37 % for 50 % effluent and 49.32 % for 100 % effluent), chlorophyll content (97.43 % for 50 % effluent and 71.05 % for 100 % effluent) and total protein content (82.63 % for 50 % effluent and 62.10 % for 100 % effluent) that leads to the reduction of total solids, total dissolved solids and total suspended solids. A. platensis with lower effluent concentration was effective than at higher concentration. Treatment with the effluent also resulted in increased activity levels of antioxidant enzymes, such as superoxide dismutase (14.58 units/g fresh weight for 50 % and 24.57 units/g fresh weight for 100 %) and catalase (0.963 units/g fresh weight for 50 % and 1.263 units/g fresh weight for 100 %). Furthermore, heavy metal content was determined using atomic absorption spectrometry. These results indicated that A. platensis has the ability to combat heavy metal stress by the induction of antioxidant enzymes demonstrating its potential usefulness in phycoremediation of tannery effluent.
本研究采用从安布尔工业区收集的受重金属污染的制革厂废水，以确定钝顶节旋藻（螺旋藻）的藻修复潜力。分析了用钝顶节旋藻处理的两种不同浓度（50%和100%）含重金属的制革厂废水的生长、吸收光谱、生化特性和抗氧化酶活性水平。废水处理显示，钝顶节旋藻的生长水平（50%废水处理时为65.37%，100%废水处理时为49.32%）、叶绿素含量（50%废水处理时为97.43%，100%废水处理时为71.05%）和总蛋白含量（50%废水处理时为82.63%，100%废水处理时为62.10%）呈剂量依赖性下降，这导致总固体、总溶解固体和总悬浮固体减少。较低废水浓度下的钝顶节旋藻比高浓度下更有效。废水处理还导致抗氧化酶活性水平增加，如超氧化物歧化酶（50%时为14.58单位/克鲜重，100%时为24.57单位/克鲜重）和过氧化氢酶（50%时为0.963单位/克鲜重，100%时为1.263单位/克鲜重）。此外，使用原子吸收光谱法测定重金属含量。这些结果表明，钝顶节旋藻具有通过诱导抗氧化酶来对抗重金属胁迫的能力，证明了其在制革厂废水藻修复中的潜在用途。
